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Literature summary for 4.6.1.13 extracted from

  • Ryan, M.; Zaikova, T.O.; Keana, J.F.W.; Goldfine, H.; Griffith, O.H.
    Listeria monocytogenes phosphatidylinositol-specific phospholipase C: Activation and allostery (2002), Biophys. Chem., 101-102, 347-358.
    View publication on PubMed
Search for more literature for 4.6.1.13

Activating Compound

Activating Compound Comment Organism Structure
dihexanoyl phosphatidylcholine activates, 4-5fold increase in catalytic efficiency, binds to a lipid-binding subsite, not to the active site, maximal activation at 0.4 mM Listeria monocytogenes

Application

Application Comment Organism
medicine PI-PLC is a virulence factor of the animal and human pathogen causing listeriosis Listeria monocytogenes

Cloned(Commentary)

Cloned (Comment) Organism
PI-PLC gene is part of the virulence gene cluster, overexpression in Escherichia coli MM294 Listeria monocytogenes

Crystallization (Commentary)

Crystallization (Comment) Organism
recombinant PI-PLC Listeria monocytogenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics, two-site kinetic model Listeria monocytogenes
0.061
-
 methyl-fluorescein myo-inositol phosphate pH 7 Listeria monocytogenes

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ MgCl2, activates Listeria monocytogenes
additional information salts have an activity-enhancing effect Listeria monocytogenes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32900
-
-
 Listeria monocytogenes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-phosphatidyl-1D-myo-inositol Listeria monocytogenes natural aggregate substrate, PI-PLC is a virulence factor of the animal and human pathogen 1D-myo-inositol 1,2-cyclic phosphate + diacylglycerol
-
 ?

Organism

Organism UniProt Comment Textmining
Listeria monocytogenes
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant PI-PLC Listeria monocytogenes

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
30
-
 pH 7.2, phosphatidylinositol as substrate, in absence of MgCl2 Listeria monocytogenes
700 1300 pH 7.2, phosphatidylinositol as substrate, in presence of MgCl2 Listeria monocytogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-phosphatidyl-1D-myo-inositol natural aggregate substrate, PI-PLC is a virulence factor of the animal and human pathogen Listeria monocytogenes 1D-myo-inositol 1,2-cyclic phosphate + diacylglycerol
-
 ?
1-phosphatidyl-1D-myo-inositol natural aggregate substrate, two-site enzyme model with interfacial cooperativity between the active site and a lipid-binding subsite, presumably adjacent to the active site Listeria monocytogenes 1D-myo-inositol 1,2-cyclic phosphate + sn-1,2-diacylglycerol
-
 ?
methyl-fluorescein myo-inositol phosphate monomeric substrate, only the D-enantiomer is active Listeria monocytogenes D-myo-inositol 1,2-cyclic phosphate + methyl-fluorescein
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
66
-
 methyl-fluorescein myo-inositol phosphate pH 7 Listeria monocytogenes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.2 assay at Listeria monocytogenes