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Literature summary for 4.6.1.13 extracted from
- Water-miscible organic cosolvents enhance phosphatidylinositol-specific phospholipase C phosphotransferase as well as phosphodiesterase activity (2003), Biochim. Biophys. Acta, 1613, 15-27.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dimethylformamide | water-miscible, enhances phosphotransferase activity | Bacillus thuringiensis |  |
| Dimethylsulfoxide | water-miscible, enhances phosphotransferase activity | Bacillus thuringiensis | |
| Isopropanol | water-miscible, maximum activation at 30%, activates regardless of the type of phosphatidylinositol substrate, enhances phosphotransferase activity | Bacillus thuringiensis | |
| additional information | isopropanol and diheptanoylphosphatidylcholine activate the hydrolytic activity towards 1D-myo-inositol 1,2-cyclic phosphate, PI-PLC exhibits kinetic interfacial activation | Bacillus thuringiensis | |
| phosphatidylcholine | activates | Bacillus thuringiensis | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli MM294 | Bacillus thuringiensis |
General Stability
| General Stability | Organism |
|---|---|
| myo-inositol stabilizes | Bacillus thuringiensis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| myo-inositol | weak | Bacillus thuringiensis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | Ca2+-independent | Bacillus thuringiensis |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| isopropanol | water/isopropanol mixture decreases stability, at 4°C protein secondary structure is stable for at least 1 week in 30% isopropanol, Tm-value for PI-PLC thermal denaturation decreases linearly with added isopropanol, in presence of 30% isopropanol the Tm-value decreases from 53.6 to 30°C, myo-inositol enhances thermostability in isopropanol | Bacillus thuringiensis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus thuringiensis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant PI-PLC | Bacillus thuringiensis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
phosphotransferase activity towards phosphatidylinositol in several aggregation states in the absence and presence of 30% isopropanol | Bacillus thuringiensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-phosphatidyl-1D-myo-inositol | aggregated substrate is preferred over monomeric substrate | Bacillus thuringiensis | 1D-myo-inositol 1,2-cyclic phosphate + diacylglycerol | a cyclic phosphodiesterase activity of PI-PLC converts 1D-myo-inositol 1,2-cyclic phosphate to inositol 1-phosphate | ? | |
| additional information | not: phosphatidylcholine | Bacillus thuringiensis | ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
some thermal unfolding of PI-PLC occurs | Bacillus thuringiensis |
| 30 | - |
Tm-value in presence of 30% isopropanol, myo-inositol enhances thermostability in isopropanol | Bacillus thuringiensis |
| 53.6 | - |
Tm-value in absence of 30% isopropanol | Bacillus thuringiensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Bacillus thuringiensis |
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