Any feedback?
Literature summary for 4.6.1.1 extracted from
- Heterodimers of adenylyl cyclases 2 and 5 show enhanced functional responses in the presence of Galpha s (2008), Cell. Signal., 20, 480-492.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| stimulating G protein | GalphaS proteins, the activation of the chimeric mutant dimer of AC2/AC5 is highly increased compared to the single isozymes, overview | Mus musculus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of fluorescent EGFP-tagged isozyme AC5, and of the fluorescence-tagged chimeric mutant dimer of AC2/AC5 in HEK-293-F cells, subcellular distribution, overview | Mus musculus |
| expression of fluorescent Luc-tagged isozyme AC2, and of the fluorescence-tagged chimeric mutant dimer of AC2/AC5 in HEK-293-F cells, subcellular distribution, overview | Mus musculus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of a chimeric mutant dimer of AC2/AC5, which shows increased activation by GalphaS proteins as compared to the single isozymes AC2 and AC5, expression of the chimeric dimer elevates cAMP production in transfected cells 6fold, while that of the single isozymes each cause a 2fold increase in cAMP production, overview | Mus musculus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| plasma membrane | integral membrane enzyme | Mus musculus | 5886 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP | Mus musculus | - |
3',5'-cyclic-AMP + diphosphate | - |
? |  |
| additional information | Mus musculus | cyclase 2 and 5 constitutively form a functional heterodimeric complex in HEK293 cells, overview | ? | - |
? | |
| additional information | Mus musculus | cyclases 2 and 5 constitutively form a functional heterodimeric complex in HEK293 cells, overview | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | P84309 | AC5 | - |
| Mus musculus | Q80TL1 | AC2 | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fibroblast | neonatal cardiac, isozyme AC2 is localized to the same structures in adult mice | Mus musculus | - |
| fibroblast | neonatal cardiac, isozyme AC5 is localized to the same structures in adult mice | Mus musculus | - |
| heart | isozyme AC2 is localized to the same structures in adult mice | Mus musculus | - |
| heart | isozyme AC5 is localized to the same structures in adult mice | Mus musculus | - |
| myocyte | ventricular, isozyme AC2 is localized to the same structures in adult mice | Mus musculus | - |
| myocyte | ventricular, isozyme AC5 is localized to the same structures in adult mice | Mus musculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Mus musculus |
| 0.000151 | - |
recombinant isozyme AC2 in HEK-293 cells | Mus musculus |
| 0.000335 | - |
recombinant isozyme AC5 in HEK-293 cells | Mus musculus |
| 0.001487 | - |
chimeric mutant dimer of AC2/AC5 | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP | - |
Mus musculus | 3',5'-cyclic-AMP + diphosphate | - |
? | |
| additional information | cyclase 2 and 5 constitutively form a functional heterodimeric complex in HEK293 cells, overview | Mus musculus | ? | - |
? | |
| additional information | cyclases 2 and 5 constitutively form a functional heterodimeric complex in HEK293 cells, overview | Mus musculus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | adenylate cyclases 2 and 5 can form a functional heterodimeric complex in HEK-293 cells | Mus musculus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AC2 | - |
Mus musculus |
| AC5 | - |
Mus musculus |
| adenylyl cyclase 2 | - |
Mus musculus |
| adenylyl cyclase 5 | - |
Mus musculus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Mus musculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Mus musculus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
