Literature summary for 4.6.1.1 extracted from

Findeisen, F.; Linder, J.U.; Schultz, A.; Schultz, J.E.; Bruegger, B.; Wieland, F.; Sinning, I.; Tews, I.
The structure of the regulatory domain of the adenylyl cyclase Rv1264 from Mycobacterium tuberculosis with bound oleic acid (2007), J. Mol. Biol., 369, 1282-1295.

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Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	regulatory domain of Rv1264 regulates AC activity in a pH-dependent manner, activated at acidic pH due to pH-dependent structural transitions of the Rv1264 dimer, strongly activated by the addition of fatty acids, oleic acid may serve as a hinge	Mycobacterium tuberculosis

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression of the histidine-tagged auto-inhibitory domain Rv1264N in Escherichia coli BL21(DE3)[pREP4] cells	Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment)	Organism
by the hanging-drop, vapour-diffusion method at 18°C, to 1.6 A resolution, crystal structure of the histidine-tagged auto-inhibitory domain Rv1264N shows the protein in a tight, disk-shaped dimer, formed around a helical bundle, and involving a protein chain crossover	Mycobacterium tuberculosis

Protein Variants

Protein Variants	Comment	Organism
E136A	shift of the pH optimum by about 0.5 unit to acidic pH	Mycobacterium tuberculosis
E136R	shift of the pH optimum by about 0.5 unit to acidic pH	Mycobacterium tuberculosis
H103A	pH regulation is barely affected, not involved in pH-regulation	Mycobacterium tuberculosis
H140A	pH regulation is barely affected, not involved in pH-regulation, shift of the pH optimum by about 0.5 unit to acidic pH	Mycobacterium tuberculosis
H140R	inhibition is relaxed slightly, with a shift of the pH optimum toward more basic values	Mycobacterium tuberculosis
H58A	pH regulation is barely affected, not involved in pH-regulation	Mycobacterium tuberculosis
additional information	deletion in the shoulder domain of the holoenzyme, linking of the two adjoining Calpha positions of residues Ala93 and His103, which are 4.6 A apart, mutant enzyme is 7fold more active at pH 8.0 compared to the wild-type enzyme, and shows only a residual 3fold activation at pH 5.5. When the entire shoulder domain is deleted, residues Asp62 to Arg105, the phenotype is similar. This deletion joins the Calpha positions of residues Gly61 and Ala106, which are 6.4 A apart in the wild-type structure	Mycobacterium tuberculosis
R132A	shift of the pH optimum by about 0.5 unit to acidic pH	Mycobacterium tuberculosis
R132E	results in a biphasic pH activity curve with a minimum at pH 6.5 and higher activity at basic pH values	Mycobacterium tuberculosis

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	addition of one or two residues results in a slightly more inhibited phenotype. Addition of three residues results in only subtle changes, probably because the insertion of three residues is compatible with an extra-helical turn. In contrast, insertion of nine amino acids almost abolishes pH sensitivity	Mycobacterium tuberculosis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mn2+	-	Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
45000	-	histidine-tagged auto-inhibitory domain Rv1264N, gel filtration	Mycobacterium tuberculosis

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	P9WQ35	-	-
Mycobacterium tuberculosis H37Rv	P9WQ35	-	-

Purification (Commentary)

Purification (Comment)	Organism
histidine-tagged auto-inhibitory domain Rv1264N, on nickel nitrilotriacetic acid/agarose column, to purity	Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP	-	Mycobacterium tuberculosis	3',5'-cAMP + diphosphate	-	?
ATP	-	Mycobacterium tuberculosis H37Rv	3',5'-cAMP + diphosphate	-	?

Subunits

Subunits	Comment	Organism
dimer	histidine-tagged auto-inhibitory domain Rv1264N, gel filtration	Mycobacterium tuberculosis

Synonyms

Synonyms	Comment	Organism
class III AC	-	Mycobacterium tuberculosis
Rv1264	-	Mycobacterium tuberculosis

pH Range

pH Minimum	pH Maximum	Comment	Organism
5	8	-	Mycobacterium tuberculosis

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Release 2023.1 (January 2023)