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Literature summary for 4.6.1.1 extracted from

  • Hammer, A.; Hodgson, D.R.; Cann, M.J.
    Regulation of prokaryotic adenylyl cyclases by CO2 (2006), Biochem. J., 396, 215-218.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
CO2
-
Anabaena sp.
CO2 stimulates 2fold at 20 mM at pH 7.5 Synechocystis sp.
additional information not activated by HCO3- Anabaena sp.
additional information not activated by HCO3- Synechocystis sp.

Cloned(Commentary)

Cloned (Comment) Organism
subcloned into pQE30, fitted with an N-terminal MRGSH6GS dodecapeptide affinity tag and expressed in Escherichia coli M15 [pREP4] cells Synechocystis sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Km value for Mn2+-ATP is higher in the presence of inorganic carbon than Cl- Synechocystis sp.
0.0114
-
ATP in the presence of Cl- Synechocystis sp.
0.0162
-
ATP in the presence of HCO3- Synechocystis sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Cl-
-
Synechocystis sp.
Mg2+
-
Synechocystis sp.
Mn2+
-
Synechocystis sp.

Organism

Organism UniProt Comment Textmining
Anabaena sp.
-
PCC 7120
-
Synechocystis sp.
-
PCC 6803
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000154
-
in presence of Mg2+ Synechocystis sp.
0.005816
-
in presence of Mn2+ Synechocystis sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP
-
Synechocystis sp. 3',5'-cAMP + diphosphate
-
?

Synonyms

Synonyms Comment Organism
Cya1
-
Synechocystis sp.
CyaB1 adenylyl cyclase
-
Anabaena sp.
Slr1991 adenylyl cyclase
-
Synechocystis sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Synechocystis sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0003
-
ATP in the presence of Cl- Synechocystis sp.
0.00045
-
ATP in the presence of HCO3- Synechocystis sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
Synechocystis sp.

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9.5
-
Synechocystis sp.