Literature summary for 4.6.1.1 extracted from

Ketkar, A.D.; Shenoy, A.R.; Ramagopal, U.A.; Visweswariah, S.S.; Suguna, K.
A Structural Basis for the Role of Nucleotide Specifying Residues in Regulating the Oligomerization of the Rv1625c Adenylyl Cyclase from M. tuberculosis (2006), J. Mol. Biol., 356, 904-916.

Search for more literature for 4.6.1.1

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment)	Organism
triple mutant K296E/F363R/D365C, 2.65 A resolution	Mycobacterium tuberculosis

Protein Variants

Protein Variants	Comment	Organism
F363R	mutant with poor adenylate cyclase acitivity	Mycobacterium tuberculosis
K296E	mutant with about 400fold reduced enzyme activity	Mycobacterium tuberculosis
K296E/D365C	double mutant with severely compromised enzyme activity	Mycobacterium tuberculosis
K296E/F363R/D365C	triple mutant with severely compromised enzyme activity	Mycobacterium tuberculosis
additional information	from Ala232 N-terminal truncated mutant has much lower enzyme activity compared with the wild type, significant reduction in the apparent Vmax with no appreciable change in the apparent K' for ATP	Mycobacterium tuberculosis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mn2+	-	Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
29000	-	2 * 29000, wild type, SDS-PAGE	Mycobacterium tuberculosis

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	P9WQ35	-	-
Mycobacterium tuberculosis H37Rv	P9WQ35	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP	-	Mycobacterium tuberculosis	cAMP + diphosphate	-	?
ATP	-	Mycobacterium tuberculosis H37Rv	cAMP + diphosphate	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 29000, wild type, SDS-PAGE	Mycobacterium tuberculosis

Synonyms

Synonyms	Comment	Organism
adenylyl cyclase	-	Mycobacterium tuberculosis
Rv1625c	-	Mycobacterium tuberculosis

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Release 2023.1 (January 2023)