Crystallization (Comment) | Organism |
---|---|
2.8 A and 2.9 A resolutions, crystal structures of the complex between stimulatory G protein alpha subunits guanosine 5'-(gamma-thio)triphosphate and the catalytic C1 and C2 domains from type V and type II adenylate cyclase, bound to forskolin and either 2'(3')-O-(N-methylanthraniloyl)-guanosine 5' triphosphate Mg2+ or 2'(3')-O-(N-methylanthraniloyl)-guanosine 5' triphosphate Mn2+ | Mammalia |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2'-d-(3')-O-(N-methylanthraniloyl)-GTP | determination of Ki values under various experimental conditions | Mammalia | |
2'-O-(N-methylanthraniloyl)-3'-deoxy-GTP | determination of Ki values under various experimental conditions | Mammalia |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
ATP | mutant VC1-A406P+IIC2-I1006V, 30°C, pH 7.4, 10 mM MnCl, 0.1 mM forskolin | Mammalia | |
0.4 | - |
ATP | recombinant form VC1-IIC2, 30°C, pH 7.4, 10 mM Mn2+ | Mammalia | |
0.43 | - |
ATP | recombinant form VC1-IIC2, 30°C, pH 7.4, 10 mM Mn2+, 0.1 mM forskolin, stimulatory G protein alpha subunits guanosine 5'-(gamma-thio)triphosphate | Mammalia | |
0.43 | - |
ATP | recombinant form VC1-IIC2, 30°C, pH 7.4, 10 mM MnCl, 0.1 mM forskolin | Mammalia | |
0.53 | - |
ATP | mutant VC1-A409P+IIC2, 30°C, pH 7.4, 10 mM MnCl, 0.1 mM forskolin | Mammalia | |
0.55 | - |
ATP | recombinant form VC1-IIC2, 30°C, pH 7.4, 10 mM Mn2+, stimulatory G protein ALPHA subunits guanosine 5'-(gamma-thio)triphosphate | Mammalia | |
0.56 | - |
ATP | recombinant form VC1-IIC2, 30°C, pH 7.4, 10 mM Mg2+, 0.1 mM forskolin | Mammalia | |
0.57 | - |
ATP | mutant VC1+IIC2-I1006V, 30°C, pH 7.4, 10 mM MnCl, 0.1 mM forskolin | Mammalia | |
0.62 | - |
ATP | recombinant form VC1-IIC2, 30°C, pH 7.4, 10 mM Mn2+, 0.1 mM forskolin | Mammalia | |
0.68 | - |
ATP | recombinant form VC1-IIC2, 30°C, pH 7.4, 10 mM Mg2+ | Mammalia | |
0.76 | - |
ATP | recombinant form VC1-IIC2, 30°C, pH 7.4, 10 mM Mg2+, stimulatory G protein alpha subunits guanosine 5'-(gamma-thio)triphosphate | Mammalia | |
0.85 | - |
ATP | recombinant form VC1-IIC2, 30°C, pH 7.4, 10 mM Mg2+, 0.1 mM forskolin, stimulatory G protein alpha subunits guanosine 5'-(gamma-thio)triphosphate | Mammalia |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mammalia | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP | - |
Mammalia | cAMP + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
adenylyl cyclase | - |
Mammalia |