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Literature summary for 4.6.1.1 extracted from

  • Chen-Goodspeed, M.; Lukan, A.N.; Dessauer, C.W.
    Modeling of Gas and Gai Regulation of Human Type V and VI Adenylyl Cyclase (2005), J. Biol. Chem., 280, 1808-1816.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
GALPHAS protein 250fold stimulation isoform V, 500fold stimulation isoform VI Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression in Sf9 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
F1078S isoform VI, mutation within the GALPHAS binding pocket Homo sapiens
N1090D isoform V, mutation within the GALPHAS binding pocket Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
GALPHAI protein inhibits basal activity of isoform V, not the basal activity of isoform VI Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
ATP wild type isoform V, 30°C Homo sapiens
0.021
-
ATP wild type isoform VI, 30°C Homo sapiens
0.026
-
ATP mutant isoform V N1090D, 30°C Homo sapiens
0.048
-
ATP mutant isoform VI F1078S, 30°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
Vmax 0.010 nmol/min/mg, wild type isoform VI, 30°C Homo sapiens
additional information
-
Vmax 0.039 nmol/min/mg, mutant isoform V N1090D, 30°C Homo sapiens
additional information
-
Vmax 0.077 nmol/min/mg, wild type isoform V, 30°C Homo sapiens
additional information
-
Vmax 0.089 nmol/min/mg, mutant isoform VI F1078S, 30°C Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP
-
Homo sapiens cAMP + diphosphate
-
?

Synonyms

Synonyms Comment Organism
adenylyl cyclase
-
Homo sapiens