Activating Compound | Comment | Organism | Structure |
---|---|---|---|
GALPHAS protein | 250fold stimulation isoform V, 500fold stimulation isoform VI | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression in Sf9 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
F1078S | isoform VI, mutation within the GALPHAS binding pocket | Homo sapiens |
N1090D | isoform V, mutation within the GALPHAS binding pocket | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GALPHAI protein | inhibits basal activity of isoform V, not the basal activity of isoform VI | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
ATP | wild type isoform V, 30°C | Homo sapiens | |
0.021 | - |
ATP | wild type isoform VI, 30°C | Homo sapiens | |
0.026 | - |
ATP | mutant isoform V N1090D, 30°C | Homo sapiens | |
0.048 | - |
ATP | mutant isoform VI F1078S, 30°C | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
Vmax 0.010 nmol/min/mg, wild type isoform VI, 30°C | Homo sapiens |
additional information | - |
Vmax 0.039 nmol/min/mg, mutant isoform V N1090D, 30°C | Homo sapiens |
additional information | - |
Vmax 0.077 nmol/min/mg, wild type isoform V, 30°C | Homo sapiens |
additional information | - |
Vmax 0.089 nmol/min/mg, mutant isoform VI F1078S, 30°C | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP | - |
Homo sapiens | cAMP + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
adenylyl cyclase | - |
Homo sapiens |