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Literature summary for 4.5.1.3 extracted from

  • Scholtz, R.; Wackett, L.P.; Egli, C.; Cook, A.M.; Leisinger, T.
    Dichloromethane dehalogenase with improved catalytic activity isolated from a fast-growing dichloromethane-utilizing bacterium (1988), J. Bacteriol., 170, 5698-5704.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.5.1.3

Activating Compound

Activating Compound Comment Organism Structure
glutathione required Hyphomicrobium sp.
glutathione required Methylorubrum extorquens
glutathione required Methylophilus sp.
glutathione Km: 0.32 mM Hyphomicrobium sp.
glutathione KM: 0.062 mM Methylorubrum extorquens
glutathione KM: 0.083 mM Methylophilus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 Dichloromethane 30°C, pH 8 Methylorubrum extorquens
0.03
-
 Dichloromethane 30°C, pH 8 Hyphomicrobium sp.
0.051
-
 Dichloromethane 30°C, pH 8 Methylophilus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 x * 33000, SDS-PAGE Hyphomicrobium sp.
34000
-
 x * 34000, SDS-PAGE Methylophilus sp.
35000
-
 x * 35000, SDS-PAGE Methylorubrum extorquens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dichloromethane + H2O Hyphomicrobium sp.
-
 formaldehyde + Cl-
-
 ?
dichloromethane + H2O Methylorubrum extorquens
-
 formaldehyde + Cl-
-
 ?
dichloromethane + H2O Methylophilus sp.
-
 formaldehyde + Cl-
-
 ?
dichloromethane + H2O Methylophilus sp. DM11
-
 formaldehyde + Cl-
-
 ?

Organism

Organism UniProt Comment Textmining
Hyphomicrobium sp.
-
-
-
Methylophilus sp. P43387
-
-
Methylophilus sp. DM11 P43387
-
-
Methylorubrum extorquens P21161
-
-

Purification (Commentary)

Purification (Comment) Organism
strain DM11 Methylophilus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dichloromethane + H2O
-
 Hyphomicrobium sp. formaldehyde + Cl-
-
 ?
dichloromethane + H2O
-
 Methylorubrum extorquens formaldehyde + Cl-
-
 ?
dichloromethane + H2O
-
 Methylophilus sp. formaldehyde + Cl-
-
 ?
dichloromethane + H2O
-
 Methylophilus sp. DM11 formaldehyde + Cl-
-
 ?

Subunits

Subunits Comment Organism
? x * 35000, SDS-PAGE Methylorubrum extorquens
? x * 34000, SDS-PAGE Methylophilus sp.
? x * 33000, SDS-PAGE Hyphomicrobium sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.55
-
 Dichloromethane 30°C, pH 8 Hyphomicrobium sp.
0.87
-
 Dichloromethane 30°C, pH 8.2 Methylorubrum extorquens
3.28
-
 Dichloromethane 30°C, pH 8.2 Methylophilus sp.