Any feedback?
Literature summary for 4.5.1.2 extracted from
- Synthesis of D-cysteine by a new pyridoxal enzyme (1983), Trends Biochem. Sci., 8, 152-153.
No PubMed abstract available
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Pseudomonas putida |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.061 | - |
3-chloro-D-alanine | - |
Pseudomonas putida |  |
| 8.1 | - |
3-chloro-D-alanine | - |
Pseudomonas putida | |
| 45 | - |
NaHS | - |
Pseudomonas putida | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
2 * 38000, SDS-PAGE | Pseudomonas putida |
| 76000 | - |
sedimentation equilibrium | Pseudomonas putida |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-chloro-D-alanine + H2O | Pseudomonas putida | - |
? | - |
? | |
| 3-chloro-D-alanine + H2O | Pseudomonas putida CR1-1 | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
CR1-1 | - |
| Pseudomonas putida CR1-1 | - |
CR1-1 | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 311 | - |
alpha,beta elimination | Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-chloro-D-alanine + H2O | - |
Pseudomonas putida | pyruvate + HCl + NH3 | - |
ir | |
| 3-chloro-D-alanine + H2O | - |
Pseudomonas putida CR1-1 | pyruvate + HCl + NH3 | - |
ir | |
| 3-chloro-D-alanine + H2O | - |
Pseudomonas putida | ? | - |
? | |
| 3-chloro-D-alanine + H2O | - |
Pseudomonas putida CR1-1 | ? | - |
? | |
| 3-chloro-D-alanine + NaHS | - |
Pseudomonas putida | D-cysteine + NaCl | - |
ir | |
| 3-chloro-D-alanine + NaHS | - |
Pseudomonas putida CR1-1 | D-cysteine + NaCl | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 38000, SDS-PAGE | Pseudomonas putida |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Pseudomonas putida |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Pseudomonas putida |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
