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Literature summary for 4.5.1.2 extracted from

  • Yamada, H.; Nagasawa, T.; Ohkishi, H.; Kawakami, B.; Tani, Y.
    Synthesis of D-cysteine from 3-chloro-D-alanine and hydrogen sulfide by 3-chloro-D-alanine hydrogen chloride-lyase (deaminating) of Pseudomonas putida (1981), Biochem. Biophys. Res. Commun., 100, 1104-1110.
    View publication on PubMed
Search for more literature for 4.5.1.2

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
8.1
-
 3-chloro-D-alanine
-
 Pseudomonas putida
46
-
 H2S
-
 Pseudomonas putida

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Pseudomonas putida 5737
-
soluble
-
 Pseudomonas putida
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-chloro-D-alanine + H2O Pseudomonas putida
-
 ?
-
 ?
3-chloro-D-alanine + H2O Pseudomonas putida CR1-1
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
 CR1-1
-
Pseudomonas putida CR1-1
-
 CR1-1
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-chloro-D-alanine + H2O
-
 Pseudomonas putida pyruvate + HCl + NH3
-
 ir
3-chloro-D-alanine + H2O
-
 Pseudomonas putida CR1-1 pyruvate + HCl + NH3
-
 ir
3-chloro-D-alanine + H2O
-
 Pseudomonas putida ?
-
 ?
3-chloro-D-alanine + H2O
-
 Pseudomonas putida CR1-1 ?
-
 ?
3-chloro-D-alanine + H2S
-
 Pseudomonas putida D-cysteine + HCl
-
 ir
3-chloro-D-alanine + H2S
-
 Pseudomonas putida CR1-1 D-cysteine + HCl
-
 ir
additional information specific for 3-chloro-D-alanine Pseudomonas putida ?
-
 ?
additional information specific for 3-chloro-D-alanine Pseudomonas putida CR1-1 ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 cysteine synthesis Pseudomonas putida

pH Range

pH Minimum pH Maximum Comment Organism
7 10
-
 Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Pseudomonas putida