Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2,4-D | activates CAS expression at 10 mM, 2,4-D is a synthetic auxin | Oryza sativa | |
auxin | upregulates CAS expression | Oryza sativa |
Cloned (Comment) | Organism |
---|---|
expressed in Arabidopsis thaliana and in Saccharomyces cerevisiae | Oryza sativa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cyanide | - |
Oryza sativa | |
ethylene | down-regulates CAS expression in etiolated rice seedlings | Oryza sativa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.27 | - |
HCN | wild type enzyme | Oryza sativa | |
0.31 | - |
HCN | recombinant enzyme from Saccharomyces cerevisiae | Oryza sativa | |
0.67 | - |
HCN | recombinant enzyme from Arabidopsis thaliana | Oryza sativa | |
0.84 | - |
L-cysteine | wild type enzyme | Oryza sativa | |
0.91 | - |
L-cysteine | recombinant enzyme from Saccharomyces cerevisiae | Oryza sativa | |
1.07 | - |
L-cysteine | recombinant enzyme from Arabidopsis thaliana | Oryza sativa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Oryza sativa | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
2 * 40000, SDS-PAGE | Oryza sativa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
anti-GFP microbead immunopurification and Mono Q colum chromatography | Oryza sativa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | - |
Oryza sativa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
13.3 | - |
wild type enzyme, after 750fold purification | Oryza sativa |
111.3 | - |
recombinant enzyme from Saccharomyces cerevisiae, after 750fold purification | Oryza sativa |
181 | - |
recombinant enzyme from Arabidopsis thaliana, after 750fold purification | Oryza sativa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-cysteine + HCN | - |
Oryza sativa | sulfide + L-3-cyanoalanine | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 40000, SDS-PAGE | Oryza sativa |
Synonyms | Comment | Organism |
---|---|---|
beta-cyanoalanine synthase | - |
Oryza sativa |
CAS | - |
Oryza sativa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Oryza sativa |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
20 | - |
recombinant enzyme from Arabidopsis thaliana | Oryza sativa | cyanide | |
40 | - |
recombinant enzyme from Saccharomyces cerevisiae | Oryza sativa | cyanide |