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Literature summary for 4.4.1.9 extracted from
- A soluble beta-cyanoalanine synthase from the gut of the variegated grasshopper Zonocerus variegatus (L.) (2007), Insect Biochem. Mol. Biol., 37, 72-79.
Application
| Application | Comment | Organism |
|---|---|---|
| agriculture | enzyme may be responsible for the ability to detoxify cyanide in insect pest and hence responsible for tolerance of the cyanogenic cassava plant, this ability can possibly be compromised by enzyme inhibition, and may lead, in the long term, to the potential use of this enzyme as drug target for pest control | Zonocerus variegatus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.39 | - |
L-cysteine | - |
Zonocerus variegatus |  |
| 6.25 | - |
NaCN | - |
Zonocerus variegatus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Zonocerus variegatus | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23250 | - |
2 * 23250, SDS-PAGE | Zonocerus variegatus |
| 552300 | - |
gel filtration | Zonocerus variegatus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Zonocerus variegatus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by ion-exchange chromatography and gel filtration | Zonocerus variegatus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| gut | - |
Zonocerus variegatus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00216 | - |
- |
Zonocerus variegatus |
| 0.0375 | - |
purified enzyme | Zonocerus variegatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-cysteine + NaCN | - |
Zonocerus variegatus | L-3-cyanoalanin + Na2S | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 23250, SDS-PAGE | Zonocerus variegatus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-CAS | - |
Zonocerus variegatus |
| beta-cyano-L-alanine synthase | - |
Zonocerus variegatus |
| beta-cyanoalanine synthase | - |
Zonocerus variegatus |
| L-cysteine: hydrogen sulphide lyase | - |
Zonocerus variegatus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Zonocerus variegatus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
activity reduces to near zero at 45°C | Zonocerus variegatus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
activity in phosphate buffer and Tris-HCl buffer | Zonocerus variegatus |
| 9 | - |
activity in borate buffer | Zonocerus variegatus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 11 | - |
Zonocerus variegatus |
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Release 2023.1 (January 2023)
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