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Literature summary for 4.4.1.9 extracted from
- beta-Cyanoalanine synthase and cysteine synthase from potato: molecular cloning, biochemical characterization, and spatial and hormonal regulation (2001), Plant Mol. Biol., 46, 749-760.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of PCAS-1 in Escherichia coli | Solanum tuberosum |
| expression of PCAS-2 in Escherichia coli | Solanum tuberosum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.13 | - |
CN- | pH 8.5 | Solanum tuberosum |  |
| 0.134 | - |
O-acetyl-L-serine | pH 7.5 | Solanum tuberosum | |
| 0.75 | - |
Na2S | pH 7.5 | Solanum tuberosum | |
| 1.5 | 2 | Na2S | pH 7.5 | Solanum tuberosum | |
| 2.14 | - |
O-acetyl-L-serine | pH 7.5 | Solanum tuberosum | |
| 2.76 | - |
L-cysteine | pH 8.5 | Solanum tuberosum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37600 | - |
x * 37600, immunoblot | Solanum tuberosum |
| 38200 | - |
x * 38200, immunoblot | Solanum tuberosum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-cysteine + HCN | Solanum tuberosum | - |
sulfide + L-3-cyanoalanine | - |
? | |
| O-acetyl-L-serine + sulfide | Solanum tuberosum | - |
L-cysteine + acetate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Solanum tuberosum | Q76MX2 | cv. Danshaku | - |
| Solanum tuberosum | Q9FS29 | cv. Danshaku | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant PCAS-1 | Solanum tuberosum |
| recombinant PCAS-2 | Solanum tuberosum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| bud | 6.2 times and 2.5 times higher levels than in tubers and leaves, respectively | Solanum tuberosum | - |
| leaf | - |
Solanum tuberosum | - |
| tuber | - |
Solanum tuberosum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.25 | - |
substrate L-cysteine | Solanum tuberosum |
| 0.43 | - |
substrate O-acetyl-L-serine | Solanum tuberosum |
| 49.5 | - |
substrate O-acetyl-L-serine | Solanum tuberosum |
| 66.2 | - |
substrate L-cysteine | Solanum tuberosum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-cysteine + HCN | - |
Solanum tuberosum | sulfide + L-3-cyanoalanine | - |
? | |
| L-cysteine + NaCN | - |
Solanum tuberosum | L-3-cyanoalanin + Na2S | - |
? | |
| O-acetyl-L-serine + sulfide | - |
Solanum tuberosum | L-cysteine + acetate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 37600, immunoblot | Solanum tuberosum |
| ? | x * 38200, immunoblot | Solanum tuberosum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PCAS-1 | - |
Solanum tuberosum |
| PCAS-2 | - |
Solanum tuberosum |
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Release 2023.1 (January 2023)
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