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Literature summary for 4.4.1.9 extracted from
- Purification and properties of beta-cyano-L-alanine synthase from the cyanide-producing bacterium Chromobacterium violaceum (1984), Biochim. Biophys. Acta, 786, 123-132.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| aminooxyacetic acid | - |
Chromobacterium violaceum |  |
| benzoic acid | - |
Chromobacterium violaceum | |
| Beta-cyano-L-alanine | - |
Chromobacterium violaceum | |
| EDTA | - |
Chromobacterium violaceum | |
| hydroxylamine | slight | Chromobacterium violaceum | |
| iodoacetamide | - |
Chromobacterium violaceum | |
| iodoacetic acid | - |
Chromobacterium violaceum | |
| KCN | high concentrations | Chromobacterium violaceum | |
| L-cysteine | high concentrations | Chromobacterium violaceum | |
| L-methionine | - |
Chromobacterium violaceum | |
| NaBH4 | - |
Chromobacterium violaceum | |
| O-acetyl-L-serine | - |
Chromobacterium violaceum | |
| p-chloromercuribenzoate | - |
Chromobacterium violaceum | |
| Semicarbazide | slight | Chromobacterium violaceum | |
| sulfhydryl reagents | - |
Chromobacterium violaceum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Chromobacterium violaceum |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | slight stimulation | Chromobacterium violaceum | |
| K+ | stimulates | Chromobacterium violaceum | |
| Zn2+ | slight stimulation | Chromobacterium violaceum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
2 * 35000, SDS-PAGE | Chromobacterium violaceum |
| 70000 | - |
gel filtration | Chromobacterium violaceum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chromobacterium violaceum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Chromobacterium violaceum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-cysteine + HCN | - |
Chromobacterium violaceum | H2S + L-3-cyanoalanine | - |
ir | |
| additional information | - |
Chromobacterium violaceum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 35000, SDS-PAGE | Chromobacterium violaceum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.15 | - |
- |
Chromobacterium violaceum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | 10.8 | - |
Chromobacterium violaceum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | 1.7 mol per mol of enzyme | Chromobacterium violaceum | |
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