Literature summary for 4.4.1.5 extracted from

Bhomkar, P.; Upadhyay, C.P.; Saxena, M.; Muthusamy, A.; Shiva Prakash, N.; Pooggin, M.; Hohn, T.; Sarin, N.B.
Salt stress alleviation in transgenic Vigna mungo L. Hepper (blackgram) by overexpression of the glyoxalase I gene using a novel Cestrum yellow leaf curling virus (CmYLCV) promoter (2008), Mol. Breed., 22, 169-181.

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Metals/Ions

Metals/Ions	Comment	Organism	Structure
methylglyoxal	10 mM, enhanced tolerance to toxic stress in transgenic Vigna mungo	Brassica juncea
NaCl	the transgenic and the untransformed plants are exposed to 100 mM NaCl. The transgenic plants survived whereas the untransformed control plants fail to survive	Brassica juncea

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
28000	-	Western blot analysis	Brassica juncea

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
methylglyoxal + glutathione	Brassica juncea	glyoxalase I is a member of the metallogltathione transferase superfamily and plays a critical role in detoxification of the cytotoxic methylglyoxal to S-D-lactoylglutathione via 1,2-hydrogen transfer	S-((R)-lactoyl)glutathione	-	?

Organism

Organism	UniProt	Comment	Textmining
Brassica juncea	-	gene gly I coding for glyoxalase I transformed into Vigna mungo L. Hepper using Agrobacterium tumefaciens, controlled by a novel constitutive Cestrum yellow leaf curling viral promotor	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
additional information	young leaves of the transgenic plant Vigna mungo, transformed with gly1 from Brassica juncae using a transformation system via Agrobacterium tumefaciens, driven by a constitutive Cestrum yellow leaf curling viral promotor	Brassica juncea	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	the GLO1 activity of the transformed Vigna mungo plants is higher than in the untransformed control plants	Brassica juncea

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
methylglyoxal + glutathione	glyoxalase I is a member of the metallogltathione transferase superfamily and plays a critical role in detoxification of the cytotoxic methylglyoxal to S-D-lactoylglutathione via 1,2-hydrogen transfer	Brassica juncea	S-((R)-lactoyl)glutathione	-	?
methylglyoxal + glutathione	non-enzymatic formation of hemithioacetal from methylglyoxal and reduced glutathione	Brassica juncea	S-((R)-lactoyl)glutathione	enzyme activity is measured spectrophotometrically as a function of thioester formation, S-((R)-lactoyl)glutathione, by measuring the rate of change of absorbance at 240 nm	?

Subunits

Subunits	Comment	Organism
?	x * 28000, Western blot analysis	Brassica juncea

Synonyms

Synonyms	Comment	Organism
Gly I	-	Brassica juncea
glyoxalase I	-	Brassica juncea

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
additional information	-	enzyme assay at room temperature	Brassica juncea

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Brassica juncea

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Release 2023.1 (January 2023)