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Literature summary for 4.4.1.5 extracted from

  • Sukdeo, N.; Clugston, S.L.; Daub, E.; Honek, J.F.
    Distinct classes of glyoxalase I: metal specificity of the Yersinia pestis, Pseudomonas aeruginosa and Neisseria meningitidis enzymes (2004), Biochem. J., 384, 111-117.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas aeruginosa
expression in Escherichia coli Neisseria meningitidis
expression in Escherichia coli Yersinia pestis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
(R)-S-lactoylglutathione pH 7.0, 0.5 mM CoCl2 Pseudomonas aeruginosa
0.016
-
methylglyoxal presence of Co2+, pH 7.0 Pseudomonas aeruginosa
0.028
-
(R)-S-lactoylglutathione pH 7.0, 0.5 mM CoCl2 Neisseria meningitidis
0.028
-
methylglyoxal presence of Co2+, pH 7.0 Neisseria meningitidis
0.029
-
(R)-S-lactoylglutathione pH 7.0, 0.5 mM CoCl2 Yersinia pestis
0.029
-
methylglyoxal presence of Co2+, pH 7.0 Yersinia pestis
0.032
-
(R)-S-lactoylglutathione pH 7.0, 0.5 mM NiCl2 Pseudomonas aeruginosa
0.032
-
methylglyoxal presence of Ni2+, pH 7.0 Pseudomonas aeruginosa
0.045
-
(R)-S-lactoylglutathione pH 7.0, 0.5 mM NiCl2 Neisseria meningitidis
0.045
-
methylglyoxal presence of Ni2+, pH 7.0 Neisseria meningitidis
0.056
-
(R)-S-lactoylglutathione pH 7.0, 0.5 mM NiCl2 Yersinia pestis
0.056
-
methylglyoxal presence of Ni2+, pH 7.0 Yersinia pestis

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ in decreasing order of activation: Ni2+, Co2+, Cd2+, Mn2+, Zn2+ Neisseria meningitidis
Cd2+ in decreasing order of activation: Ni2+, Co2+, Mn2+, Cd2+ Pseudomonas aeruginosa
Cd2+ in decreasing order of activation: Ni2+, Co2+, Mn2+, Cd2+ Yersinia pestis
Cd2+ increased enzyme activity Pseudomonas aeruginosa
Cd2+ increased enzyme activity Neisseria meningitidis
Cd2+ increased enzyme activity Yersinia pestis
Co2+ in decreasing order of activation: Ni2+, Co2+, Cd2+, Mn2+, Zn2+ Neisseria meningitidis
Co2+ in decreasing order of activation: Ni2+, Co2+, Mn2+, Cd2+ Pseudomonas aeruginosa
Co2+ in decreasing order of activation: Ni2+, Co2+, Mn2+, Cd2+ Yersinia pestis
Co2+ increased enzyme activity Pseudomonas aeruginosa
Co2+ increased enzyme activity Neisseria meningitidis
Co2+ increased enzyme activity Yersinia pestis
Mn2+ in decreasing order of activation: Ni2+, Co2+, Cd2+, Mn2+, Zn2+ Neisseria meningitidis
Mn2+ in decreasing order of activation: Ni2+, Co2+, Mn2+, Cd2+ Pseudomonas aeruginosa
Mn2+ in decreasing order of activation: Ni2+, Co2+, Mn2+, Cd2+ Yersinia pestis
Mn2+ increased enzyme activity Pseudomonas aeruginosa
Mn2+ increased enzyme activity Neisseria meningitidis
Mn2+ increased enzyme activity Yersinia pestis
additional information Mg2+, Ca2+, Zn2+ no increase in enzyme activity Pseudomonas aeruginosa
additional information Mg2+, Ca2+, Zn2+ no increase in enzyme activity Neisseria meningitidis
additional information Mg2+, Ca2+, Zn2+ no increase in enzyme activity Yersinia pestis
Ni2+ in decreasing order of activation: Ni2+, Co2+, Cd2+, Mn2+, Zn2+ Neisseria meningitidis
Ni2+ in decreasing order of activation: Ni2+, Co2+, Mn2+, Cd2+ Pseudomonas aeruginosa
Ni2+ in decreasing order of activation: Ni2+, Co2+, Mn2+, Cd2+ Yersinia pestis
Ni2+ increased enzyme activity Pseudomonas aeruginosa
Ni2+ increased enzyme activity Neisseria meningitidis
Ni2+ increased enzyme activity Yersinia pestis
Zn2+ in decreasing order of activation: Ni2+, Co2+, Cd2+, Mn2+, Zn2+ Neisseria meningitidis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
14250
-
electrospray ionization mass spectrometry Pseudomonas aeruginosa
14251
-
x * 14251, electrospray ionization mass spectrometry, x * 14251, calculated Pseudomonas aeruginosa
14833
-
x * 14833, electrospray ionization mass spectrometry, x * 14834, calculated Yersinia pestis
14834
-
x * 14833, electrospray ionization mass spectrometry, x * 14834, calculated Yersinia pestis
15669
-
x * 15669, electrospray ionization mass spectrometry, x * 15669, calculated Neisseria meningitidis
15670
-
electrospray ionization mass spectrometry Neisseria meningitidis
29670
-
electrospray ionization mass spectrometry Yersinia pestis
148333
-
2 * 148333, electrospray ionization mass spectrometry Yersinia pestis

Organism

Organism UniProt Comment Textmining
Neisseria meningitidis
-
-
-
Pseudomonas aeruginosa
-
-
-
Yersinia pestis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas aeruginosa
-
Neisseria meningitidis
-
Yersinia pestis
recombinant enzyme Pseudomonas aeruginosa
recombinant enzyme Neisseria meningitidis
recombinant enzyme Yersinia pestis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
140
-
presence of Co2+, pH 7.0 Yersinia pestis
180
-
presence of Co2+, pH 7.0 Pseudomonas aeruginosa
279
-
presence of Co2+, pH 7.0 Neisseria meningitidis
390
-
micromol/min/mg protein, Vmax (R)-S-lactoylglutathione, pH 7.0, 0.5 mM NiCl2, 279 micromol/min/mg protein Vmax (R)-S-lactoylglutathione, pH 7.0, 0.5 mM CoCl2 Neisseria meningitidis
390
-
presence of Ni2+, pH 7.0 Neisseria meningitidis
571
-
micromol/min/mg protein, Vmax (R)-S-lactoylglutathione, pH 7.0, 0.5 mM NiCl2, 180 micromol/min/mg protein Vmax (R)-S-lactoylglutathione, pH 7.0, 0.5 mM CoCl2 Pseudomonas aeruginosa
571
-
presence of Ni2+, pH 7.0 Pseudomonas aeruginosa
618
-
micromol/min/mg protein, Vmax (R)-S-lactoylglutathione, pH 7.0, 0.5 mM NiCl2, 140 micromol/min/mg protein Vmax (R)-S-lactoylglutathione, pH 7.0, 0.5 mM CoCl2 Yersinia pestis
618
-
presence of Ni2+, pH 7.0 Yersinia pestis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-S-lactoylglutathione
-
Pseudomonas aeruginosa glutathione + methylglyoxal
-
?
(R)-S-lactoylglutathione
-
Neisseria meningitidis glutathione + methylglyoxal
-
?
(R)-S-lactoylglutathione
-
Yersinia pestis glutathione + methylglyoxal
-
?
glutathione + methylglyoxal
-
Pseudomonas aeruginosa S-lactoylglutathione
-
?
glutathione + methylglyoxal
-
Neisseria meningitidis S-lactoylglutathione
-
?
glutathione + methylglyoxal
-
Yersinia pestis S-lactoylglutathione
-
?

Subunits

Subunits Comment Organism
? x * 14251, electrospray ionization mass spectrometry, x * 14251, calculated Pseudomonas aeruginosa
? x * 14833, electrospray ionization mass spectrometry, x * 14834, calculated Yersinia pestis
? x * 15669, electrospray ionization mass spectrometry, x * 15669, calculated Neisseria meningitidis
dimer 2 * 148333, electrospray ionization mass spectrometry Yersinia pestis

Synonyms

Synonyms Comment Organism
GLXI
-
Pseudomonas aeruginosa
GLXI
-
Neisseria meningitidis
GLXI
-
Yersinia pestis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
69
-
methylglyoxal presence of Co2+, pH 7.0 Yersinia pestis
86
-
methylglyoxal presence of Co2+, pH 7.0 Pseudomonas aeruginosa
146
-
methylglyoxal presence of Co2+, pH 7.0 Neisseria meningitidis
204
-
methylglyoxal presence of Ni2+, pH 7.0 Neisseria meningitidis
271
-
methylglyoxal presence of Ni2+, pH 7.0 Pseudomonas aeruginosa
306
-
methylglyoxal presence of Ni2+, pH 7.0 Yersinia pestis