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Literature summary for 4.4.1.4 extracted from

  • Lancaster, J.E.; Shaw, M.L.; Joyce, M.D.; McCallum, J.A.; McManus, M.T.
    A novel alliinase from onion roots. Biochemical characterization and cDNA cloning (2000), Plant Physiol., 122, 1269-1279.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.4.1.4

Cloned(Commentary)

Cloned (Comment) Organism
-
 Allium cepa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2
-
 cystine alliinase I Allium cepa
2.6
-
 S-propenyl-L-Cys sulfoxide alliinase I Allium cepa
3
-
 S-propenyl-L-Cys sulfoxide alliinase II Allium cepa
9.7
-
 cystine alliinase II Allium cepa
16.3
-
 S-propyl-L-Cys sulfoxide alliinase II Allium cepa
18.3
-
 S-allyl-L-Cys sulfoxide alliinase I Allium cepa
22
-
 S-propyl-L-Cys sulfoxide alliinase I Allium cepa
24.1
-
 S-allyl-L-Cys sulfoxide alliinase II Allium cepa
45.7
-
 S-ethyl-L-Cys sulfoxide alliinase I Allium cepa
47
-
 S-methyl-L-Cys sulfoxide alliinase I Allium cepa
68.4
-
 S-ethyl-L-Cys sulfoxide alliinase II Allium cepa
96.6
-
 S-methyl-L-Cys sulfoxide alliinase II Allium cepa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51000
-
 alliinase I, gel filtration Allium cepa
52700
-
 1 * 52700, alliinase I, SDS-PAGE Allium cepa
57500
-
 alliinase II, gel filtration Allium cepa

Organism

Organism UniProt Comment Textmining
Allium cepa
-
-
-
Leucocoryne angustipetala
-
-
-
Leucocoryne coquimbensis
-
-
-
Leucocoryne odorata
-
-
-
Leucocoryne purpurea
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein alliinase I and alliinase II differ in glycosylation. Both contain xylose/fucose containing complex-type N-linked glycans, and alliinase II also contains terminal mannose structures Allium cepa

Purification (Commentary)

Purification (Comment) Organism
-
 Allium cepa

Source Tissue

Source Tissue Comment Organism Textmining
bulb
-
 Leucocoryne angustipetala
-
bulb
-
 Leucocoryne purpurea
-
bulb only in Leucocoryne odorata
-
leaf
-
 Leucocoryne angustipetala
-
leaf
-
 Leucocoryne purpurea
-
leaf only in Allium cepa
-
leaf only in Leucocoryne coquimbensis
-
root
-
 Allium cepa
-
scape
-
 Leucocoryne angustipetala
-
scape
-
 Leucocoryne purpurea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
119.7
-
-
 Allium cepa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-S-propyl-L-cysteine sulfoxide
-
 Allium cepa propane-1-sulfenic acid + pyruvate + NH3
-
 ?
(+)-S-propyl-L-cysteine sulfoxide
-
 Leucocoryne angustipetala propane-1-sulfenic acid + pyruvate + NH3
-
 ?
(+)-S-propyl-L-cysteine sulfoxide
-
 Leucocoryne purpurea propane-1-sulfenic acid + pyruvate + NH3
-
 ?
(+)-S-propyl-L-cysteine sulfoxide
-
 Leucocoryne coquimbensis propane-1-sulfenic acid + pyruvate + NH3
-
 ?
(+)-S-propyl-L-cysteine sulfoxide
-
 Leucocoryne odorata propane-1-sulfenic acid + pyruvate + NH3
-
 ?
cystine
-
 Allium cepa ?
-
 ?
S-allyl-L-Cys sulfoxide + H2O
-
 Allium cepa 2-propenesulfenic acid + pyruvate + NH3
-
 ?
S-ethyl-L-Cys sulfoxide + H2O
-
 Allium cepa ethanesulfenic acid + pyruvate + NH3
-
 ?
S-methyl-L-Cys sulfoxide
-
 Allium cepa methanesulfenic acid + pyruvate + NH3
-
 ?
S-propenyl-L-Cys sulfoxide
-
 Allium cepa ?
-
 ?
S-propyl-L-Cys sulfoxide
-
 Allium cepa propanesulfenic acid + pyruvate + NH4+
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 52700, alliinase I, SDS-PAGE Allium cepa
monomer 1 * 57500, alliinase II, SDS-PAGE Allium cepa

Synonyms

Synonyms Comment Organism
alliinase I
-
 Allium cepa
alliinase II
-
 Allium cepa

pI Value

Organism Comment pI Value Maximum pI Value
Allium cepa alliinase I, isoelectric focusing 4.7 4.6
Allium cepa alliinase II, isoelectric focusing
-
 6.9