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Literature summary for 4.4.1.4 extracted from

  • Manabe, T.; Hasumi, A.; Sugiyama, M.; Yamazaki, M.; Saito, K.
    Alliinase [S-alk(en)yl-L-cysteine sulfoxide lyase] from Allium tuberosum (Chinese chive): purification, localization, cDNA cloning and heterologous functional expression (1998), Eur. J. Biochem., 257, 21-30.
    View publication on PubMed
Search for more literature for 4.4.1.4

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli and Saccharomyces cerevisiae Allium tuberosum

Protein Variants

Protein Variants Comment Organism
K280A almost no detectable alliinase activity Allium tuberosum
K280R almost no detectable alliinase activity Allium tuberosum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7
-
 S-ethyl-L-Cys sulfoxide
-
 Allium tuberosum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 x * 48000, SDS-PAGE Allium tuberosum
54083
-
 x * 54083, calculation from nucleotide sequence Allium tuberosum
67000
-
 gel filtration Allium tuberosum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Allium tuberosum the enzyme is responsible for the degradative conversion of S-alk(en)yl-L-Cys sulfoxide to volatile odorous sulfur-containing metabolites ?
-
 ?

Organism

Organism UniProt Comment Textmining
Allium tuberosum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Allium tuberosum

Source Tissue

Source Tissue Comment Organism Textmining
shoot the enzyme predominantly accumulates in the bundle sheath cells Allium tuberosum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
152.8
-
-
 Allium tuberosum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 S-ethyl-L-Cys sulfoxide + H2O
-
 Allium tuberosum ethyl ethanethiosulfinate + 2 pyruvate + 2 NH3
-
 ?
additional information the enzyme is responsible for the degradative conversion of S-alk(en)yl-L-Cys sulfoxide to volatile odorous sulfur-containing metabolites Allium tuberosum ?
-
 ?

Subunits

Subunits Comment Organism
? x * 48000, SDS-PAGE Allium tuberosum
? x * 54083, calculation from nucleotide sequence Allium tuberosum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7 sodium phosphate buffer Allium tuberosum
8 8.5 Tris/HCl buffer Allium tuberosum

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Allium tuberosum