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Literature summary for 4.4.1.4 extracted from

  • Nock, L.P.; Mazelis, M.
    The C-S lyases of higher plants: preparation and properties of homogeneous alliin lyase from garlic (Allium sativum) (1986), Arch. Biochem. Biophys., 249, 27-33.
    View publication on PubMed
Search for more literature for 4.4.1.4

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.3
-
 S-allyl-L-Cys sulfoxide
-
 Allium sativum
5.7
-
 S-ethyl-L-Cys sulfoxide
-
 Allium sativum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 2 * 42000, gel filtration on a column equilibrated with 6 M urea Allium sativum
85000
-
 gel filtration Allium sativum

Organism

Organism UniProt Comment Textmining
Allium sativum
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification glycoprotein Allium sativum
side-chain modification contains 5.5% carbohydrate, 40% as pentose Allium sativum

Purification (Commentary)

Purification (Comment) Organism
-
 Allium sativum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
126
-
-
 Allium sativum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 S-ethyl-L-Cys sulfoxide + H2O
-
 Allium sativum ethyl ethanethiosulfinate + 2 pyruvate + 2 NH3
-
 ?
S-allyl-L-cysteine sulfoxide + H2O
-
 Allium sativum 2-propene-thioic acid + pyruvate + NH4+ + H+
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 42000, gel filtration on a column equilibrated with 6 M urea Allium sativum

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate contains one mol of pyridoxal 5'-phosphate per mol of subunit Allium sativum
pyridoxal 5'-phosphate enzyme contains pyridoxal 5'-phosphate Allium sativum