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Literature summary for 4.4.1.35 extracted from

  • Ramirez, E.C.; Whitaker, J.R.
    Biochemical characterization of cystine lyase from broccoli (Brassica oleracea var. italica) (1999), J. Agric. Food Chem., 47, 2218-2225.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
cyanide 12.5 mM, 5% residual activity Brassica oleracea var. italica
DL-homocysteine 2.5 mM, 59% residual activity Brassica oleracea var. italica
hydroxylamine 12.5 mM, 3% residual activity Brassica oleracea var. italica
L-cysteine linear noncompetitive inhibitor Brassica oleracea var. italica
pyridoxal 2.5 mM, 63% residual activity Brassica oleracea var. italica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0812
-
L-cystine pH 8.4, 30°C Brassica oleracea var. italica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
-
Brassica oleracea var. italica
48000
-
-
Brassica oleracea var. italica
94000
-
MALDI-TOF Brassica oleracea var. italica
100000 110000 gel filtration Brassica oleracea var. italica

Organism

Organism UniProt Comment Textmining
Brassica oleracea var. italica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Brassica oleracea var. italica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.7
-
pH 8.4, 30°C Brassica oleracea var. italica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-cysteine S-sulfate + H2O
-
Brassica oleracea var. italica ?
-
?
L-cystine + H2O
-
Brassica oleracea var. italica L-thiocysteine + pyruvate + NH3 overall reaction ?
L-djenkolic acid + H2O 18-24% of the activity with L-cystine Brassica oleracea var. italica ?
-
?
additional information negligible activity with L-cystathionine Brassica oleracea var. italica ?
-
?
S-ethyl L-cysteine + H2O 71% of the activity with L-cystine Brassica oleracea var. italica ?
-
?
S-methyl L-cysteine + H2O 29% of the activity with L-cystine Brassica oleracea var. italica ?
-
?
S-propyl L-cysteine + H2O 29% of the activity with L-cystine Brassica oleracea var. italica ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 47000, MALDI-TOF, 2 * 48000, SDS-PAGE Brassica oleracea var. italica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
Brassica oleracea var. italica

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
10 min, stable Brassica oleracea var. italica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.1
-
-
Brassica oleracea var. italica

pH Stability

pH Stability pH Stability Maximum Comment Organism
3
-
complete loss of activity Brassica oleracea var. italica
4 8 30°C, stable for 24 h Brassica oleracea var. italica

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Brassica oleracea var. italica

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.5
-
L-cysteine pH 8.4, 30°C Brassica oleracea var. italica