Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.4.1.34 extracted from

  • Van Hylckama Vlieg, J.; Kingma, J.; Van Den Wijngaard, A.; Janssen, D.
    A glutathione S-transferase with activity towards cis-1,2-dichloroepoxyethane is involved in isoprene utilization by Rhodococcus sp. strain AD45 (1998), Appl. Environ. Microbiol., 64, 2800-2805.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibitory: 1 mM MnCl2, CoCl2, CuSO4, MgSO4, RbCl2, or ZnSO4 Rhodococcus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
cis-1,2-dichloroepoxyethane pH 8.5, 30°C Rhodococcus sp.
0.3
-
(3R)-3,4-epoxy-3-methylbut-1-ene pH 8.5, 30°C Rhodococcus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
2 * 27000, SDS-PAGE Rhodococcus sp.
46000
-
gel filtration Rhodococcus sp.

Organism

Organism UniProt Comment Textmining
Rhodococcus sp. Q9RBP4
-
-

Storage Stability

Storage Stability Organism
4°C or -20°C, storable for 3 months with less than 20% loss of activity Rhodococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(3R)-3,4-epoxy-3-methylbut-1-ene + glutathione i.e. isoprene monoxide. Nucleophilic attack of GSH occurs on the tertiary carbon atom of the epoxide ring Rhodococcus sp. 2-(glutathion-S-yl)-2-methylbut-3-en-1-ol i.e. 1-hydroxy-2-glutathionyl-2-methyl-3-butene ?
1,2-epoxybutane + glutathione 26% of the activity with (3R)-3,4-epoxy-3-methylbut-1-ene Rhodococcus sp. 2-(glutathion-S-yl)-butan-1-ol
-
?
1,2-epoxyhexane + glutathione 25% of the activity with (3R)-3,4-epoxy-3-methylbut-1-ene Rhodococcus sp. 2-(glutathion-S-yl)-hexan-1-ol
-
?
cis-1,2-dichloroepoxyethane + glutathione 4% of the activity with (3R)-3,4-epoxy-3-methylbut-1-ene Rhodococcus sp. 1,2-dichloro-2-(glutathion-S-yl)-ethan-1-ol
-
?
cis-2,3-epoxybutane + glutathione 13% of the activity with (3R)-3,4-epoxy-3-methylbut-1-ene Rhodococcus sp. 3-(glutathion-S-yl)-butan-2-ol
-
?
epibromohydrin + glutathione 25% of the activity with (3R)-3,4-epoxy-3-methylbut-1-ene Rhodococcus sp. 3-bromo-2-(glutathion-S-yl)-propan-1-ol
-
?
epichlorohydrin + glutathione 31% of the activity with (3R)-3,4-epoxy-3-methylbut-1-ene Rhodococcus sp. 3-chloro-2-(glutathion-S-yl)-propan-1-ol
-
?
epifluorohydrin + glutathione 28% of the activity with (3R)-3,4-epoxy-3-methylbut-1-ene Rhodococcus sp. 3-fluoro-2-(glutathion-S-yl)-propan-1-ol
-
?
epithiopropane + glutathione 34% of the activity with (3R)-3,4-epoxy-3-methylbut-1-ene Rhodococcus sp. 2-(glutathion-S-yl)-propanthiol
-
?
epoxyethane + glutathione 37% of the activity with (3R)-3,4-epoxy-3-methylbut-1-ene Rhodococcus sp. 2-(glutathion-S-yl)-ethan-1-ol
-
?
epoxypropane + glutathione 32% of the activity with (3R)-3,4-epoxy-3-methylbut-1-ene Rhodococcus sp. 2-(glutathion-S-yl)-propan-1-ol
-
?

Subunits

Subunits Comment Organism
dimer 2 * 27000, SDS-PAGE Rhodococcus sp.