Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibitory: 1 mM MnCl2, CoCl2, CuSO4, MgSO4, RbCl2, or ZnSO4 | Rhodococcus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
cis-1,2-dichloroepoxyethane | pH 8.5, 30°C | Rhodococcus sp. | |
0.3 | - |
(3R)-3,4-epoxy-3-methylbut-1-ene | pH 8.5, 30°C | Rhodococcus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
2 * 27000, SDS-PAGE | Rhodococcus sp. |
46000 | - |
gel filtration | Rhodococcus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus sp. | Q9RBP4 | - |
- |
Storage Stability | Organism |
---|---|
4°C or -20°C, storable for 3 months with less than 20% loss of activity | Rhodococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3R)-3,4-epoxy-3-methylbut-1-ene + glutathione | i.e. isoprene monoxide. Nucleophilic attack of GSH occurs on the tertiary carbon atom of the epoxide ring | Rhodococcus sp. | 2-(glutathion-S-yl)-2-methylbut-3-en-1-ol | i.e. 1-hydroxy-2-glutathionyl-2-methyl-3-butene | ? | |
1,2-epoxybutane + glutathione | 26% of the activity with (3R)-3,4-epoxy-3-methylbut-1-ene | Rhodococcus sp. | 2-(glutathion-S-yl)-butan-1-ol | - |
? | |
1,2-epoxyhexane + glutathione | 25% of the activity with (3R)-3,4-epoxy-3-methylbut-1-ene | Rhodococcus sp. | 2-(glutathion-S-yl)-hexan-1-ol | - |
? | |
cis-1,2-dichloroepoxyethane + glutathione | 4% of the activity with (3R)-3,4-epoxy-3-methylbut-1-ene | Rhodococcus sp. | 1,2-dichloro-2-(glutathion-S-yl)-ethan-1-ol | - |
? | |
cis-2,3-epoxybutane + glutathione | 13% of the activity with (3R)-3,4-epoxy-3-methylbut-1-ene | Rhodococcus sp. | 3-(glutathion-S-yl)-butan-2-ol | - |
? | |
epibromohydrin + glutathione | 25% of the activity with (3R)-3,4-epoxy-3-methylbut-1-ene | Rhodococcus sp. | 3-bromo-2-(glutathion-S-yl)-propan-1-ol | - |
? | |
epichlorohydrin + glutathione | 31% of the activity with (3R)-3,4-epoxy-3-methylbut-1-ene | Rhodococcus sp. | 3-chloro-2-(glutathion-S-yl)-propan-1-ol | - |
? | |
epifluorohydrin + glutathione | 28% of the activity with (3R)-3,4-epoxy-3-methylbut-1-ene | Rhodococcus sp. | 3-fluoro-2-(glutathion-S-yl)-propan-1-ol | - |
? | |
epithiopropane + glutathione | 34% of the activity with (3R)-3,4-epoxy-3-methylbut-1-ene | Rhodococcus sp. | 2-(glutathion-S-yl)-propanthiol | - |
? | |
epoxyethane + glutathione | 37% of the activity with (3R)-3,4-epoxy-3-methylbut-1-ene | Rhodococcus sp. | 2-(glutathion-S-yl)-ethan-1-ol | - |
? | |
epoxypropane + glutathione | 32% of the activity with (3R)-3,4-epoxy-3-methylbut-1-ene | Rhodococcus sp. | 2-(glutathion-S-yl)-propan-1-ol | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 27000, SDS-PAGE | Rhodococcus sp. |