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Literature summary for 4.4.1.32 extracted from
- Oligomeric structure, enzyme kinetics, and substrate specificity of the phycocyanin alpha subunit phycocyanobilin lyase (1994), J. Biol. Chem., 269, 8686-8694.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| phycoerythrobilin | competitive inhibitor of (2R,3E)-phycocyanobilin addition | Synechococcus elongatus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000024 | - |
(2R,3E)-phycocyanobilin | pH 8.0, 37°C | Synechococcus elongatus | |
| 0.0108 | - |
apo-[C-phycocyanin alpha-subunit] | pH 8.0, 37°C | Synechococcus elongatus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 22000 | - |
1 * 29000 (CpcE) + 1 * 22000 (CpcF), SDS-PAGE; CpcE and CpcF form an enzymatically active 1:1 complex | Synechococcus elongatus |
| 29000 | - |
1 * 29000 (CpcE) + 1 * 22000 (CpcF), SDS-PAGE; CpcE and CpcF form an enzymatically active 1:1 complex | Synechococcus elongatus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Synechococcus elongatus | Q44115 | Q44115: subunit alpha (cpcE), Q44116: phycobilisome maturation protein (cpcF) | - |
| Synechococcus elongatus PCC 7942 | Q44115 | Q44115: subunit alpha (cpcE), Q44116: phycobilisome maturation protein (cpcF) | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant CpcE and CpcF proteins | Synechococcus elongatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| apo-[C-phycocyanin alpha-subunit] + (2R,3E)-phycocyanobilin | the enzyme shows a preference for phycocyanobilin relative to phycoerythrobilin, both in binding affinity and in the rate of catalysis, sufficient to account for selective attachment of phycocyanobilin to the apo C-phycocyanin alpha-subunit | Synechococcus elongatus | [C-phycocyanin alpha-subunit]-Cys84-phycocyanobilin | - |
? | |
| apo-[C-phycocyanin alpha-subunit] + (2R,3E)-phycocyanobilin | the enzyme shows a preference for phycocyanobilin relative to phycoerythrobilin, both in binding affinity and in the rate of catalysis, sufficient to account for selective attachment of phycocyanobilin to the apo C-phycocyanin alpha-subunit | Synechococcus elongatus PCC 7942 | [C-phycocyanin alpha-subunit]-Cys84-phycocyanobilin | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 29000 (CpcE) + 1 * 22000 (CpcF), SDS-PAGE; CpcE and CpcF form an enzymatically active 1:1 complex | Synechococcus elongatus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CpcEF | - |
Synechococcus elongatus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Synechococcus elongatus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.025 | - |
(2R,3E)-phycocyanobilin | pH 8.0, 37°C | Synechococcus elongatus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Synechococcus elongatus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00086 | - |
phycoerythrobilin | pH 8.0, 37°C | Synechococcus elongatus | |
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