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Literature summary for 4.4.1.28 extracted from

  • Xie, Y.; Lai, D.; Mao, Y.; Zhang, W.; Shen, W.; Guan, R.
    Molecular cloning, characterization, and expression analysis of a novel gene encoding L-cysteine desulfhydrase from Brassica napus (2013), Mol. Biotechnol., 54, 737-746.
    View publication on PubMed
Search for more literature for 4.4.1.28

Cloned(Commentary)

Cloned (Comment) Organism
expression as a His-tagged fusion protein in Escherichia coli Brassica napus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 L-cysteine pH 9.0, 37°C Brassica napus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34500
-
 x * 34500, calculated from sequence Brassica napus
35000
-
 x * 35000, SDS-PAGE Brassica napus

Organism

Organism UniProt Comment Textmining
Brassica napus J9WJN8
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Brassica napus

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
 Brassica napus
-
root from seedling Brassica napus
-
seedling root Brassica napus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.066
-
 pH 9.0, 37°C, purified enzyme Brassica napus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-cysteine + H2O
-
 Brassica napus sulfide + NH3 + pyruvate
-
 ?

Subunits

Subunits Comment Organism
? x * 35000, SDS-PAGE Brassica napus
? x * 34500, calculated from sequence Brassica napus

Synonyms

Synonyms Comment Organism
At5g28030
-
 Brassica napus
DES1
-
 Brassica napus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Brassica napus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Brassica napus

pH Range

pH Minimum pH Maximum Comment Organism
8 9.5 pH 8.0: about 30% of maximal activity, pH 9.5: about 65% of maximal activity Brassica napus

pI Value

Organism Comment pI Value Maximum pI Value
Brassica napus assay at
-
 5.2

Expression

Organism Comment Expression
Brassica napus BnDES1 expression is significantly down-regulated by cadmium down
Brassica napus abscisic acid and gibberellic acid moderately induce the expression of the enzyme (BnDES1), and the maximal inducible time points are 24 h and 48 h, separately. Kinetin and indole-3-acetic acid induce the BnDES1 transcripts, with the same time point of 12 h for the maximal induction. BnDES1 transcripts are gradually up-regulated during 24 h or 12 h treatment periods by sodium nitroprusside or salicylic acid, and then fall back or keep the higher level until 48 h, respectively. The addition of paraquat brings about the rapid and moderated induction of BnDES1 during 3 h treatment period. BnDES1 transcript is induced sharply by the NaHS and L-cysteine treatments, both with maximum induction at 12 h following treatments up

General Information

General Information Comment Organism
metabolism the enzyme is responsible for the H2S production, and may play an important role in plant growth regulators and chemical stimuli responses Brassica napus