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Literature summary for 4.4.1.28 extracted from

  • Chu, L.; Ebersole, J.L.; Kurzban, G.P.; Holt, S.C.
    Cystalysin, a 46-kDa L-cysteine desulfhydrase from Treponema denticola: biochemical and biophysical characterization (1999), Clin. Infect. Dis., 28, 442-450.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 0.5 mM, enhances activity Treponema denticola

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in Escherichia coli LC-67 Treponema denticola

Inhibitors

Inhibitors Comment Organism Structure
dithiothreitol 0.5 mM Treponema denticola
glycine
-
Treponema denticola
L-alanine
-
Treponema denticola
L-asparagine less effective inhibitor, Ki-value above 150 mM Treponema denticola
L-methionine less effective inhibitor, Ki-value above 150 mM Treponema denticola
L-serine
-
Treponema denticola
L-threonine less effective inhibitor, Ki-value above 150 mM Treponema denticola
L-valine less effective inhibitor, Ki-value above 150 mM Treponema denticola
additional information not inhibited by Nalpha-p-tosyl-L-lysine chloromethyl ketone, pronase, proteinase K, CaCl2, MgCl2, ZnCl2, PMSF or benzamidine Treponema denticola

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4
-
L-cysteine pH 7.4, 22°C Treponema denticola

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
x * 46000, SDS-PAGE Treponema denticola

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-cysteine + H2O Treponema denticola
-
sulfide + NH3 + pyruvate
-
?
L-cysteine + H2O Treponema denticola LC-67
-
sulfide + NH3 + pyruvate
-
?
L-cysteine + H2O Treponema denticola ATCC 35404
-
sulfide + NH3 + pyruvate
-
?

Organism

Organism UniProt Comment Textmining
Treponema denticola
-
-
-
Treponema denticola ATCC 35404
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Treponema denticola

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cystathionine + H2O
-
Treponema denticola NH3 + pyruvate + homocysteine
-
?
cystathionine + H2O
-
Treponema denticola LC-67 NH3 + pyruvate + homocysteine
-
?
cystathionine + H2O
-
Treponema denticola ATCC 35404 NH3 + pyruvate + homocysteine
-
?
L-cysteine + H2O
-
Treponema denticola sulfide + NH3 + pyruvate
-
?
L-cysteine + H2O
-
Treponema denticola LC-67 sulfide + NH3 + pyruvate
-
?
L-cysteine + H2O
-
Treponema denticola ATCC 35404 sulfide + NH3 + pyruvate
-
?
S-aminoethyl-L-cysteine + H2O
-
Treponema denticola NH3 + pyruvate + cysteamine
-
?

Subunits

Subunits Comment Organism
? x * 46000, SDS-PAGE Treponema denticola

Synonyms

Synonyms Comment Organism
cystalysin
-
Treponema denticola
L-cysteine desulfhydrase
-
Treponema denticola

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
assay at Treponema denticola

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
significant decrease in enzymatic activity above 50°C Treponema denticola
70
-
30 min, complete inactivation Treponema denticola

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
12
-
L-cysteine pH 7.4, 22°C Treponema denticola

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Treponema denticola

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
7.4
-
glycine pH 7.4, 22°C Treponema denticola
15
-
L-serine pH 7.4, 22°C Treponema denticola
22
-
L-alanine pH 7.4, 22°C Treponema denticola

General Information

General Information Comment Organism
physiological function the enzyme could function in vivo as a virulence factor Treponema denticola