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Literature summary for 4.4.1.23 extracted from

  • Jin, Y.O.; Cheung, S.; Coleman, N.V.; Mattes, T.E.
    Association of missense mutations in epoxyalkane coenzyme M transferase with adaptation of Mycobacterium sp. strain JS623 to growth on vinyl chloride (2010), Appl. Environ. Microbiol., 76, 3413-3419.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.4.1.23

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli and Mycobacterium smegmatis Mycobacterium sp. JS623

Protein Variants

Protein Variants Comment Organism
additional information mutations developed during vinyl chloride adaptations Mycobacterium sp. JS623
R257L higher enzyme activities compared to wild-type, growth on vinyl chloride more rapidly than the wild-type strain Mycobacterium sp. JS623
W243G higher enzyme activities compared to wild-type, growth on vinyl chloride more rapidly than the wild-type strain Mycobacterium sp. JS623

Organism

Organism UniProt Comment Textmining
Mycobacterium sp. JS623 B9VTP0 variant JS 623-E and JS 623-T
-
Mycobacterium sp. JS623 B9VTP5 variant JS 623-E and JS 623-T
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.039
-
 wild-type enzyme, transferred to Mycobacterium smegmatis Mycobacterium sp. JS623
0.056
-
 mutation W243G, transferred to Mycobacterium smegmatis Mycobacterium sp. JS623
0.062
-
 mutation R257L, transferred to Mycobacterium smegmatis Mycobacterium sp. JS623
0.074
-
 wild-type enzyme Mycobacterium sp. JS623
0.15
-
 vinyl-chloride adapted variant JS623-E Mycobacterium sp. JS623
0.645
-
 vinyl-chloride adapted variant JS623-T Mycobacterium sp. JS623

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
epoxyethane + CoM assay at pH 8.0, 30°C Mycobacterium sp. JS623 2-hydroxyethyl-CoM
-
 ?

Synonyms

Synonyms Comment Organism
EaCoMT
-
 Mycobacterium sp. JS623
epoxyalkane coenzyme M transferase
-
 Mycobacterium sp. JS623

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Mycobacterium sp. JS623

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Mycobacterium sp. JS623