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Literature summary for 4.4.1.21 extracted from

  • Song, X.D.; Liu, C.J.; Huang, S.H.; Li, X.R.; Yang, E.; Luo, Y.Y.
    Cloning, expression and characterization of two S-ribosylhomocysteine lyases from Lactobacillus plantarum YM-4-3 Implication of conserved and divergent roles in quorum sensing (2018), Protein Expr. Purif., 145, 32-38 .
    View publication on PubMed
Search for more literature for 4.4.1.21

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Lactiplantibacillus plantarum

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 1 mM, 6.1% of initial activity, respectively; 1 mM, 7.5% of initial activity, respectively Lactiplantibacillus plantarum
glycerol 1 mM, 0.1% of initial activity, respectively; 1 mM, 8% of initial activity, respectively Lactiplantibacillus plantarum
Ni2+ 1 mM, 23% of initial activity, respectively; 1 mM, 7.8% of initial activity, respectively Lactiplantibacillus plantarum
Tween-20 1 mM, 85% of initial activity, respectively; 1 mM, 86% of initial activity, respectively Lactiplantibacillus plantarum
Zn2+ 1 mM, 19.8% of initial activity, respectively; 1 mM, 19% of initial activity, respectively Lactiplantibacillus plantarum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.88
-
 S-ribosylhomocysteine isoform LuxS2, pH 8.0, 37°C Lactiplantibacillus plantarum
1.23
-
 S-ribosylhomocysteine isoform LuxS1, pH 8.0, 45°C Lactiplantibacillus plantarum

Metals/Ions

Metals/Ions Comment Organism Structure
Al3+ 1 mM, 110% of initial activity, respectively Lactiplantibacillus plantarum
Al3+ 1 mM, 115% of initial activity, respectively Lactiplantibacillus plantarum
Ca2+ 1 mM, 108% of initial activity, respectively Lactiplantibacillus plantarum
Ca2+ 1 mM, 19% of initial activity, respectively Lactiplantibacillus plantarum
EDTA 1 mM, 113% of initial activity, respectively Lactiplantibacillus plantarum
EDTA 1 mM, 123% of initial activity, respectively Lactiplantibacillus plantarum
Fe2+ 1 mM, 110% of initial activity, respectively Lactiplantibacillus plantarum
Fe2+ 1 mM, 99% of initial activity, respectively Lactiplantibacillus plantarum
Mn2+ 1 mM, 103% of initial activity, respectively Lactiplantibacillus plantarum
Mn2+ 1 mM, 114% of initial activity, respectively Lactiplantibacillus plantarum

Organism

Organism UniProt Comment Textmining
Lactiplantibacillus plantarum ETF12584 isoform LuxS1
-
Lactiplantibacillus plantarum ETF12641 isoform LuxS2
-
Lactiplantibacillus plantarum YM-4-3 ETF12584 isoform LuxS1
-
Lactiplantibacillus plantarum YM-4-3 ETF12641 isoform LuxS2
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information both isoforms LuxS1 and LuxS2 can effectively convert S-ribosylhomocysteine to autoinducer AI-2 and homocysteine, and they may have redundant functions Lactiplantibacillus plantarum ?
-
 ?
additional information both isoforms LuxS1 and LuxS2 can effectively convert S-ribosylhomocysteine to autoinducer AI-2 and homocysteine, and they may have redundant functions Lactiplantibacillus plantarum YM-4-3 ?
-
 ?
S-ribosylhomocysteine
-
 Lactiplantibacillus plantarum L-homocysteine + 4,5-dihydroxy-2,3-pentanedione
-
 ?
S-ribosylhomocysteine
-
 Lactiplantibacillus plantarum YM-4-3 L-homocysteine + 4,5-dihydroxy-2,3-pentanedione
-
 ?

Subunits

Subunits Comment Organism
? x * 17400, calculated from sequence, x * 22000, SDS-PAGE, recombinant protein including His-tag Lactiplantibacillus plantarum
? x * 17500, calculated from sequence, x * 22000, SDS-PAGE, recombinant protein including His-tag Lactiplantibacillus plantarum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 isoform LuxS2 Lactiplantibacillus plantarum
45
-
 isoform LuxS1 Lactiplantibacillus plantarum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.34
-
 S-ribosylhomocysteine isoform LuxS2, pH 8.0, 37°C Lactiplantibacillus plantarum
0.47
-
 S-ribosylhomocysteine isoform LuxS1, pH 8.0, 45°C Lactiplantibacillus plantarum

pI Value

Organism Comment pI Value Maximum pI Value
Lactiplantibacillus plantarum isoform LuxS1, calculated from sequence
-
 6.3
Lactiplantibacillus plantarum isoform LuxS2, calculated from sequence
-
 6.3