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Literature summary for 4.4.1.20 extracted from

  • Ahmad, S.; Ytterberg, A.J.; Thulasingam, M.; Tholander, F.; Bergman, T.; Zubarev, R.; Wetterholm, A.; Rinaldo-Matthis, A.; Haeggstroem, J.Z.
    Phosphorylation of leukotriene C4 synthase at serine 36 impairs catalytic activity (2016), J. Biol. Chem., 291, 18410-18418 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.4.1.20

Crystallization (Commentary)

Crystallization (Comment) Organism
comparative molecular dynamic analysis. Residue Ser36 upon phosphorylation will pull the first luminal loop of LTC4S toward the neighboring subunit of the functional homotrimer, thereby forming hydrogen bonds with catalytic residue Arg104 in the adjacent subunit. The phosphorylation-induced interaction leads to a reduction of the catalytic activity Homo sapiens

Protein Variants

Protein Variants Comment Organism
S36E phosphomimetic mutant, displays about 20 % of wild-type activity. Mutant shows poor lipid substrate binding Homo sapiens
T40E phosphomimetic mutant, activity is hardly affected Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
 glutathione wild-type, pH 7.8, 23°C Homo sapiens
0.076
-
 leukotriene A4 wild-type, pH 7.8, 23°C Homo sapiens
0.085
-
 leukotriene A4 mutant T40E, pH 7.8, 23°C Homo sapiens
0.12
-
 glutathione mutant S36E, pH 7.8, 23°C Homo sapiens
0.22
-
 glutathione mutant T40E, pH 7.8, 23°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q16873
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein residue Ser36 is the major p70S6k phosphorylation site, along with a low frequency site at Thr40 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
leukotriene A4 + glutathione
-
 Homo sapiens leukotriene C4
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3
-
 glutathione mutant S36E, pH 7.8, 23°C Homo sapiens
12
-
 glutathione mutant T40E, pH 7.8, 23°C Homo sapiens
19
-
 glutathione wild-type, pH 7.8, 23°C Homo sapiens
62
-
 leukotriene A4 mutant T40E, pH 7.8, 23°C Homo sapiens
105
-
 leukotriene A4 wild-type, pH 7.8, 23°C Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
26
-
 glutathione mutant S36E, pH 7.8, 23°C Homo sapiens
50
-
 glutathione mutant T40E, pH 7.8, 23°C Homo sapiens
130
-
 leukotriene A4 mutant S36E, pH 7.8, 23°C Homo sapiens
270
-
 glutathione wild-type, pH 7.8, 23°C Homo sapiens
770
-
 leukotriene A4 mutant T40E, pH 7.8, 23°C Homo sapiens
1390
-
 leukotriene A4 wild-type, pH 7.8, 23°C Homo sapiens