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Literature summary for 4.4.1.2 extracted from

  • El-Sayed, A.S.; Khalaf, S.A.; Aziz, H.A.
    Characterization of homocysteine gamma-lyase from submerged and solid cultures of Aspergillus fumigatus ASH (JX006238) (2013), J. Microbiol. Biotechnol., 23, 499-510.
    View publication on PubMed
Search for more literature for 4.4.1.2

Activating Compound

Activating Compound Comment Organism Structure
pyridoxal 5'-phosphate
-
 Aspergillus fumigatus

Application

Application Comment Organism
medicine the enzyme displays a strong affinity to homocysteine, followed by methionine and cysteine when compared with non-S amino acids, confirming its potency against homocysteinuria related diseases, and as an anti-cardiovascular agent and a specific biosensor for homocysteinuria Aspergillus fumigatus

Protein Variants

Protein Variants Comment Organism
up induction of the enzyme is mainly sulfur amino-acid-dependent, especially methionine and homocysteine. The induction of the enzyme in response to various amino acids is evaluated, and the results reveal that sulfur-containing amino acids strongly induce Hcy gamma-lyase production by the isolate when compared with other amino acids. The maximum enzyme productivity is detected in the presence of methionine (1.8 U/mg), homocysteine (1.2 U/mg), cysteine (1.23 U/mg), and asparagine (1.2 U/mg). Some enzyme activity is also detected when using various non-sulfur amino acids as substrates, such as glutamine, alanine, and phenylalanine, indicating the multifunctional deaminating catalytic activity of the crude enzyme. The lowest enzyme activity is detected in the presence of tyrosine and lysine Aspergillus fumigatus
up the influence of different carbon sources (0.05-0.2%) on the enzyme is assessed when using 100 mM L-methionine as the substrate. From the data, the highest enzyme yield is obtained with 0.15% sucrose (2.54 U/mg), followed by glucose (2.18 U/mg), representing about 1.42- and 1.2-fold increments, respectively, over the control (1.8 U/mg). The enzyme productivity increases about 12.7fold when using 0.15% sucrose over a carbon-free medium Aspergillus fumigatus

Inhibitors

Inhibitors Comment Organism Structure
dithiothreitol 10 mM, 86% loss of activity Aspergillus fumigatus
Hg2+ 10 mM, 95% loss of activity Aspergillus fumigatus
hydroxylamine 10 mM, 89% loss of activity Aspergillus fumigatus
KCl 10 mM, 11% loss of activity Aspergillus fumigatus
additional information no inhibition by EDTA Aspergillus fumigatus
SDS 10 mM, 78% loss of activity Aspergillus fumigatus
Tween 80 1 mM, about 40% loss of activity Aspergillus fumigatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.46
-
 L-homocysteine pH 7.0, 35°C Aspergillus fumigatus
4.1
-
 L-methionine pH 7.0, 35°C Aspergillus fumigatus
4.9
-
 L-cysteine pH 7.0, 35°C Aspergillus fumigatus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Aspergillus fumigatus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 x * 50000, SDS-PAGE Aspergillus fumigatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-homocysteine + H2O Aspergillus fumigatus
-
 hydrogen sulfide + NH3 + 2-oxobutanoate
-
 ?
L-homocysteine + H2O Aspergillus fumigatus ASH JX006238
-
 hydrogen sulfide + NH3 + 2-oxobutanoate
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus fumigatus
-
-
-
Aspergillus fumigatus ASH JX006238
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aspergillus fumigatus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.48
-
 pH 7.0, 35°C, solid state fermentation, purified enzyme Aspergillus fumigatus
16.6
-
 pH 7.0, 35°C, submerged fermentation, purified enzyme Aspergillus fumigatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycine + H2O 26.3% of the oxo-acid release activity compared to L-homocysteine as substrate Aspergillus fumigatus ?
-
 ?
glycine + H2O 26.3% of the oxo-acid release activity compared to L-homocysteine as substrate Aspergillus fumigatus ASH JX006238 ?
-
 ?
L-alanine + H2O 32.1% of the oxo-acid release activity compared to L-homocysteine as substrate Aspergillus fumigatus ?
-
 ?
L-alanine + H2O 32.1% of the oxo-acid release activity compared to L-homocysteine as substrate Aspergillus fumigatus ASH JX006238 ?
-
 ?
L-asparagine + H2O 33.3% of the oxo-acid release activity compared to L-homocysteine as substrate Aspergillus fumigatus ?
-
 ?
L-asparagine + H2O 33.3% of the oxo-acid release activity compared to L-homocysteine as substrate Aspergillus fumigatus ASH JX006238 ?
-
 ?
L-cysteine + H2O 43.3% of the oxo-acid release activity compared to L-homocysteine as substrate Aspergillus fumigatus 2-oxopropanoate + NH3 + ?
-
 ?
L-cystine + H2O 16.2% of the oxo-acid release activity compared to L-homocysteine as substrate Aspergillus fumigatus ?
-
 ?
L-cystine + H2O 16.2% of the oxo-acid release activity compared to L-homocysteine as substrate Aspergillus fumigatus ASH JX006238 ?
-
 ?
L-homocysteine + H2O
-
 Aspergillus fumigatus hydrogen sulfide + NH3 + 2-oxobutanoate
-
 ?
L-homocysteine + H2O
-
 Aspergillus fumigatus ASH JX006238 hydrogen sulfide + NH3 + 2-oxobutanoate
-
 ?
L-lysine + H2O 27.1% of the oxo-acid release activity compared to L-homocysteine as substrate Aspergillus fumigatus ?
-
 ?
L-methionine + H2O 90% of the oxo-acid release compared to L-homocysteine as substrate Aspergillus fumigatus 2-oxobutanoate + NH3 + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 50000, SDS-PAGE Aspergillus fumigatus

Synonyms

Synonyms Comment Organism
Hyc gamma-lyase
-
 Aspergillus fumigatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37 40
-
 Aspergillus fumigatus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 T1/2: 18.1 h, at 30°C Aspergillus fumigatus
35
-
 T1/2: 9.9 h, at 30°C Aspergillus fumigatus
40
-
 stable below, T1/2: 5.9 h, at 30°C Aspergillus fumigatus
50
-
 T1/2: 3.3 h, at 30°C Aspergillus fumigatus
60
-
 T1/2: 1.9 h, at 30°C Aspergillus fumigatus
70
-
 Tm-value is 70.1°C. Retention of about 50% of its initial activity when heated for 60 min at 70.1°C Aspergillus fumigatus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00077
-
 L-cysteine pH 7.0, 35°C Aspergillus fumigatus
0.00097
-
 L-methionine pH 7.0, 35°C Aspergillus fumigatus
0.00139
-
 L-homocysteine pH 7.0, 35°C Aspergillus fumigatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
 Aspergillus fumigatus

pH Stability

pH Stability pH Stability Maximum Comment Organism
3
-
 4°C, 2 h, 50% reduction in enzyme activity Aspergillus fumigatus
6.5 7.8 maximal structural and catalytic stability within the pH-range 6.5-7.8 Aspergillus fumigatus
10.4
-
 4°C, 2 h, 20% reduction in enzyme activity Aspergillus fumigatus

pI Value

Organism Comment pI Value Maximum pI Value
Aspergillus fumigatus isoelectric focusing, detected by pH precipitation with no glycosyl residues
-
 5