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Literature summary for 4.4.1.19 extracted from

  • Partovi, S.E.; Mus, F.; Gutknecht, A.E.; Martinez, H.A.; Tripet, B.P.; Lange, B.M.; DuBois, J.L.; Peters, J.W.
    Coenzyme M biosynthesis in bacteria involves phosphate elimination by a functionally distinct member of the aspartase/fumarase superfamily (2018), J. Biol. Chem., 293, 5236-5246 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21(DE3) Xanthobacter autotrophicus

Crystallization (Commentary)

Crystallization (Comment) Organism
homology modeling of structure. The conserved residue Ser-285B may feasibly initiate the general base-catalyzed reaction through abstraction of the phosphosulfolactate Cbeta proton with stabilization from Lys-291B and putative H-bond donors present in the binding site Xanthobacter autotrophicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphoenolpyruvate + sulfite Xanthobacter autotrophicus the enzyme is involved in the biosynthesis of coenzyme M 2-O-phospho-3-sulfolactate
-
?

Organism

Organism UniProt Comment Textmining
Xanthobacter autotrophicus
-
-
-
Xanthobacter autotrophicus Q9AF21
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Xanthobacter autotrophicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
487
-
pH 8.0, 30°C Xanthobacter autotrophicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + sulfite
-
Xanthobacter autotrophicus 2-O-phospho-3-sulfolactate
-
?
phosphoenolpyruvate + sulfite the enzyme is involved in the biosynthesis of coenzyme M Xanthobacter autotrophicus 2-O-phospho-3-sulfolactate
-
?
phosphoenolpyruvate + sulfite
-
Xanthobacter autotrophicus (2R)-2-O-phospho-3-sulfolactate
-
?

Synonyms

Synonyms Comment Organism
XAUT_RS24680 gene name, gene is located on 320-kb linear megaplasmid pXAUT01 Xanthobacter autotrophicus
Xcb1
-
Xanthobacter autotrophicus
XcbB1
-
Xanthobacter autotrophicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Xanthobacter autotrophicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Xanthobacter autotrophicus

General Information

General Information Comment Organism
metabolism the enzyme is involved in the biosynthesis of coenzyme M Xanthobacter autotrophicus