Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21(DE3) | Xanthobacter autotrophicus |
Crystallization (Comment) | Organism |
---|---|
homology modeling of structure. The conserved residue Ser-285B may feasibly initiate the general base-catalyzed reaction through abstraction of the phosphosulfolactate Cbeta proton with stabilization from Lys-291B and putative H-bond donors present in the binding site | Xanthobacter autotrophicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + sulfite | Xanthobacter autotrophicus | the enzyme is involved in the biosynthesis of coenzyme M | 2-O-phospho-3-sulfolactate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xanthobacter autotrophicus | - |
- |
- |
Xanthobacter autotrophicus | Q9AF21 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Xanthobacter autotrophicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
487 | - |
pH 8.0, 30°C | Xanthobacter autotrophicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + sulfite | - |
Xanthobacter autotrophicus | 2-O-phospho-3-sulfolactate | - |
? | |
phosphoenolpyruvate + sulfite | the enzyme is involved in the biosynthesis of coenzyme M | Xanthobacter autotrophicus | 2-O-phospho-3-sulfolactate | - |
? | |
phosphoenolpyruvate + sulfite | - |
Xanthobacter autotrophicus | (2R)-2-O-phospho-3-sulfolactate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
XAUT_RS24680 | gene name, gene is located on 320-kb linear megaplasmid pXAUT01 | Xanthobacter autotrophicus |
Xcb1 | - |
Xanthobacter autotrophicus |
XcbB1 | - |
Xanthobacter autotrophicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Xanthobacter autotrophicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Xanthobacter autotrophicus |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in the biosynthesis of coenzyme M | Xanthobacter autotrophicus |