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Literature summary for 4.4.1.17 extracted from

  • Nicholson, D.W.; Köhler, H.; Neupert, W.
    Import of cytochrome c into mitochondria. Cytochrome c heme lyase (1987), Eur. J. Biochem., 164, 147-157.
    View publication on PubMed
Search for more literature for 4.4.1.17

Activating Compound

Activating Compound Comment Organism Structure
Cytosolic factor requirement Neurospora crassa
glutathione activation Neurospora crassa
additional information independent of a potential across the inner mitochondrial membrane generated by N,N,N',N'-tetramethyl-p-phenylenediamine/ascorbate Neurospora crassa
NADH 10fold activation between 1 and 10 mM of NADH Neurospora crassa
NADPH about half of the activation observed with NADH Neurospora crassa

Inhibitors

Inhibitors Comment Organism Structure
Deuterohemin r; reversible inhibition Neurospora crassa
hemin at concentrations higher than 0.002 mM; at higher concentrations Neurospora crassa
N-ethylmaleimide kinetics, in vivo; pretreatment of reticulocyte lysates with N-ethylmaleimide results in nearly complete inactivation Neurospora crassa

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrial inner membrane
-
 Neurospora crassa 5743
-
mitochondrion intermembrane space Neurospora crassa 5739
-
soluble
-
 Neurospora crassa
-
-

Organism

Organism UniProt Comment Textmining
Neurospora crassa
-
 wild-type strain 74A
-

Purification (Commentary)

Purification (Comment) Organism
partial Neurospora crassa
partial, subfractionation of mitochondria with digitonin Neurospora crassa

Source Tissue

Source Tissue Comment Organism Textmining
hypha
-
 Neurospora crassa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 activity is independent of a potential across the inner mitochondrial membrane generated by N,N,N',N'-tetramethyl-p-phenylenediamine/ascorbate, requires an unidentified cytosolic factor Neurospora crassa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Apocytochrome c + heme catalyzes covalent attachment of heme group to two cysteine residues in the protein Neurospora crassa Holocytochrome c
-
 ?
additional information distinct from apocytochrome c binding protein Neurospora crassa ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
 Neurospora crassa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Neurospora crassa

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8.5 about half-maximal activity at pH 5.5 and 8.5 Neurospora crassa

Cofactor

Cofactor Comment Organism Structure
NADH activation Neurospora crassa
NADPH activation Neurospora crassa