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Literature summary for 4.4.1.16 extracted from
- A nifS-like gene, csdB, encodes an Escherichia coli counterpart of mammalian selenocysteine lyase. Gene cloning, purification, characterization and preliminary X-ray crystallographic studies (1999), J. Biol. Chem., 274, 14768-14772.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli JM109 | Escherichia coli |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging drop vapor diffusion method | Escherichia coli |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
SDS-PAGE | Escherichia coli |
| 44440 | - |
calculated from amino acid sequence | Escherichia coli |
| 88000 | - |
gel filtration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-selenocysteine + reduced acceptor | Escherichia coli | probably functions as selenide delivery system | selenide + L-alanine + acceptor | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
stain K-12 ICR130 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| overexpressed protein | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5.6 | - |
purified enzyme, pH 7.5, 37°C | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-cysteine sulfinic acid + reduced acceptor | approx. 15% of activity with L-selenocysteine | Escherichia coli | SO2 + L-alanine + acceptor | - |
? | |
| L-selenocysteine + reduced acceptor | - |
Escherichia coli | selenide + L-alanine + acceptor | - |
? | |
| L-selenocysteine + reduced acceptor | probably functions as selenide delivery system | Escherichia coli | selenide + L-alanine + acceptor | - |
? | |
| additional information | traces of activity with L-cysteine, L-selenocystine, L-cystine and L-aspartic acid | Escherichia coli | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 43000, SDS-PAGE | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | two other proteins (CDS and IscS) with activity towards L-selenocysteine identified that are much less specific | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7.5 | - |
Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | required | Escherichia coli | |
| pyridoxal 5'-phosphate | 1 mol bound per mol enzyme (subunit) | Escherichia coli | |
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