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Literature summary for 4.4.1.15 extracted from

  • Riemenschneider, A.; Wegele, R.; Schmidt, A.; Papenbrock, J.
    Isolation and characterization of a D-cysteine desulfhydrase protein from Arabidopsis thaliana (2005), FEBS J., 272, 1291-1304.
    View publication on PubMed
Search for more literature for 4.4.1.15

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Arabidopsis thaliana

General Stability

General Stability Organism
glycerol, pyridoxal-5'-phosphate, dithiothreitol and EDTA do not increase the stability of the protein after freezing Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
aminooxy acetic acid at 10 microM, IC50 value is 0.0305 mM Arabidopsis thaliana
hydroxylamine at 10 microM, IC50 value is 0.0159 mM Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.25
-
 D-cysteine purified recombinant mature protein Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Arabidopsis thaliana 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41700
-
 monomer, mature protein without N-terminal extension, calculated from amino acid sequence Arabidopsis thaliana
42000
-
 x * 42000, SDS-PAGE, unprocessed protein Arabidopsis thaliana
43000
-
 x * 43000, SDS-PAGE, mature protein Arabidopsis thaliana
43900
-
 monomer, calculated from amino acid sequence Arabidopsis thaliana
44000
-
 x * 44000, SDS-PAGE, post-translationally modified protein Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-cysteine + H2O Arabidopsis thaliana
-
 sulfide + NH3 + pyruvate
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
 ecotype C24
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-cysteine + H2O
-
 Arabidopsis thaliana sulfide + NH3 + pyruvate
-
 ?
D-cysteine + H2O 37°C, pH 8.0 Arabidopsis thaliana sulfide + NH3 + pyruvate
-
 ?
L-cysteine + H2O 5% activity compared to D-cysteine Arabidopsis thaliana sulfide + NH3 + pyruvate
-
 ?

Subunits

Subunits Comment Organism
? x * 42000, SDS-PAGE, unprocessed protein Arabidopsis thaliana
? x * 43000, SDS-PAGE, mature protein Arabidopsis thaliana
? x * 44000, SDS-PAGE, post-translationally modified protein Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
D-CDes
-
 Arabidopsis thaliana
D-cysteine desulfhydrase
-
 Arabidopsis thaliana

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
additional information
-
 loss of activity at 50°C, no activity at 60°C. The enzyme is also very sensitive to freezing. One freeze-thaw cycle leads to a loss of activity of 75%. Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6
-
 D-cysteine
-
 Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Arabidopsis thaliana

pI Value

Organism Comment pI Value Maximum pI Value
Arabidopsis thaliana mature protein without N-terminal extension, calculated from amino acid sequence
-
 4.34
Arabidopsis thaliana calculated from amino acid sequence
-
 7.2

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0159
-
 at 10 microM, IC50 value is 0.0159 mM Arabidopsis thaliana hydroxylamine
0.0305
-
 at 10 microM, IC50 value is 0.0305 mM Arabidopsis thaliana aminooxy acetic acid