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Literature summary for 4.4.1.15 extracted from

  • Schmidt, A.
    D-cysteine desulfhydrase from spinach (1987), Methods Enzymol., 143, 449-453.
No PubMed abstract available
Search for more literature for 4.4.1.15

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol probably by preventing autooxidation of substrate Spinacia oleracea

Inhibitors

Inhibitors Comment Organism Structure
aminooxyacetic acid
-
 Spinacia oleracea
Co2+
-
 Spinacia oleracea
hydroxylamine
-
 Spinacia oleracea
Ni2+
-
 Spinacia oleracea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-cysteine + H2O Spinacia oleracea plant sulfur metabolism sulfide + NH3 + pyruvate additional product suggested ?
D-cysteine + H2O Spinacia oleracea plant sulfur metabolism sulfide + NH3 + pyruvate more sulfide than pyruvate and ammonium formed ?

Organism

Organism UniProt Comment Textmining
Spinacia oleracea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Spinacia oleracea

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.01
-
 purified enzyme, 37°C, pH 9.0 Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-cysteine + H2O very specific, no activity with L-cysteine, mercaptoacetic acid, mercaptoethanol, D-cystine, L-cystine, cysteamine, L-cysteine methylester and dithioerythritol Spinacia oleracea sulfide + NH3 + pyruvate additional product suggested ?
D-cysteine + H2O very specific, no activity with L-cysteine, mercaptoacetic acid, mercaptoethanol, D-cystine, L-cystine, cysteamine, L-cysteine methylester and dithioerythritol Spinacia oleracea sulfide + NH3 + pyruvate more sulfide than pyruvate and ammonium formed ?
D-cysteine + H2O plant sulfur metabolism Spinacia oleracea sulfide + NH3 + pyruvate additional product suggested ?
D-cysteine + H2O plant sulfur metabolism Spinacia oleracea sulfide + NH3 + pyruvate more sulfide than pyruvate and ammonium formed ?
additional information L-cysteine is not utilized Spinacia oleracea ?
-
 ?

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Spinacia oleracea