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Literature summary for 4.4.1.14 extracted from

  • Zhou, H.; Wang, H.W.; Zhu, K.; Sui, S.F.; Xu, P.; Yang, S.F.; Li, N.
    The multiple roles of conserved arginine 286 of 1-aminocyclopropane-1-carboxylate synthase. Coenzyme binding, substrate binding, and beyond (1999), Plant Physiol., 121, 913-919.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.4.1.14

Cloned(Commentary)

Cloned (Comment) Organism
expression of C-terminal deletion mutant in Escherichia coli Solanum lycopersicum

Protein Variants

Protein Variants Comment Organism
R286L low affinity for both pyridoxal 5'-phosphate and S-adenosyl-L-methionine, 8000fold decrease in overall catalytic activity, i.e. kcat/Km Solanum lycopersicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
 S-adenosyl-L-methionine
-
 Solanum lycopersicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine Solanum lycopersicum
-
 1-aminocyclopropane-1-carboxylate + methylthioadenosine
-
 ?

Organism

Organism UniProt Comment Textmining
Solanum lycopersicum
-
 isoenzyme ACS2
-

Purification (Commentary)

Purification (Comment) Organism
recombinant ACC synthase Solanum lycopersicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine
-
 Solanum lycopersicum 1-aminocyclopropane-1-carboxylate + methylthioadenosine
-
 ?

Synonyms

Synonyms Comment Organism
ACC synthase
-
 Solanum lycopersicum
LE-ACS2
-
 Solanum lycopersicum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.052 2.1 S-adenosyl-L-methionine
-
 Solanum lycopersicum
9.79
-
 S-adenosyl-L-methionine
-
 Solanum lycopersicum

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Solanum lycopersicum