Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.4.1.14 extracted from

  • McCarthy, D.L.; Capitani, G.; Feng, L.; Gruetter, M.G.; Kirsch, J.F.
    Glutamate 47 in 1-aminocyclopropane-1-carboxylate synthase is a major specificity determinant (2001), Biochemistry, 40, 12276-12284.
    View publication on PubMed
Search for more literature for 4.4.1.14

Cloned(Commentary)

Cloned (Comment) Organism
expression of ACC synthase in Escherichia coli Malus domestica

Protein Variants

Protein Variants Comment Organism
E47D 3.8% of wild-type ACC synthase activity Malus domestica
E47Q 0.9% of wild-type ACC synthase activity Malus domestica

Inhibitors

Inhibitors Comment Organism Structure
L-Vinylglycine mechanism-based inactivation Malus domestica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.026
-
 (S,S)-S-adenosylmethionine alpha,gamma-elimination Malus domestica
0.037
-
 (R,S)-S-adenosylmethionine beta,gamma-elimination Malus domestica
0.27
-
 L-Vinylglycine deamination Malus domestica
37
-
 alanine transamination Malus domestica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine Malus domestica
-
 1-aminocyclopropane-1-carboxylate + methylthioadenosine
-
 ?

Organism

Organism UniProt Comment Textmining
Malus domestica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant ACC synthase Malus domestica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R,S)-S-adenosyl-L-methionine
-
 Malus domestica vinylglycine + methylthioadenosine
-
 ?
(S,S)-S-adenosyl-L-methionine
-
 Malus domestica 1-aminocyclopropane-1-carboxylate + methylthioadenosine
-
 ?
L-vinylglycine
-
 Malus domestica alpha-ketobutyrate + ammonia
-
 ?
pyridoxal 5'-phosphate + alanine
-
 Malus domestica pyridoxamine 5'-phosphate + pyruvate
-
 ?
S-adenosyl-L-methionine
-
 Malus domestica 1-aminocyclopropane-1-carboxylate + methylthioadenosine
-
 ?

Synonyms

Synonyms Comment Organism
ACC synthase
-
 Malus domestica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.019
-
 alanine transamination Malus domestica
0.079
-
 (R,S)-S-adenosylmethionine beta,gamma-elimination Malus domestica
0.7 1 L-Vinylglycine deamination Malus domestica
0.71
-
 L-Vinylglycine deamination Malus domestica
18
-
 (S,S)-S-adenosylmethionine alpha,gamma-elimination Malus domestica

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Malus domestica