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Literature summary for 4.4.1.14 extracted from
- L-Vinylglycine is an alternative substrate as well as a mechanism-based inhibitor of 1-aminocyclopropane-1-carboxylate synthase (2000), Biochemistry, 39, 2436-2444.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression of C-terminally truncated ACC synthase in Pichia pastoris | Malus domestica |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| L-Vinylglycine | approx. 60% loss of activity after 5 min, approx. 90% loss of activity after 40 min, biphasic inativation, mechanism based inhibition | Malus domestica |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.4 | - |
L-Vinylglycine | - |
Malus domestica | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine | Malus domestica | rate-determining step in the biosynthesis of ethylene | 1-aminocyclopropane-1-carboxylate + methylthioadenosine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Malus domestica | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant ACC synthase | Malus domestica |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 6900 | - |
- |
Malus domestica |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, truncated ACC synthase, at least 6 months, no loss of activity | Malus domestica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-vinylglycine | - |
Malus domestica | alpha-ketobutyrate + ammonia | - |
? | |
| S-adenosyl-L-methionine | - |
Malus domestica | 1-aminocyclopropane-1-carboxylate + methylthioadenosine | - |
? | |
| S-adenosyl-L-methionine | rate-determining step in the biosynthesis of ethylene | Malus domestica | 1-aminocyclopropane-1-carboxylate + methylthioadenosine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ACC synthase | - |
Malus domestica |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.8 | - |
L-Vinylglycine | - |
Malus domestica | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Malus domestica | |
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Release 2023.1 (January 2023)
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