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Literature summary for 4.4.1.14 extracted from
- Purification and properties of 1-aminocyclopropane-1-carboxylate synthase of mesocarp of Cucurbita maxima Duch. fruits (1986), Plant Cell Physiol., 27, 969-980.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| aminoethoxyvinylglycine | - |
Cucurbita maxima |  |
| aminooxyacetic acid | - |
Cucurbita maxima | |
| Cu2+ | - |
Cucurbita maxima | |
| homocysteine | slight | Cucurbita maxima | |
| S-adenosyl-L-methionine | in presence of pyridoxal 5'-phosphate | Cucurbita maxima | |
| S-adenosylhomocysteine | - |
Cucurbita maxima | |
| Semicarbazide | - |
Cucurbita maxima | |
| Zn2+ | - |
Cucurbita maxima | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0133 | - |
S-adenosyl-L-methionine | - |
Cucurbita maxima | |
| 0.12 | - |
S-adenosyl-L-methionine | - |
Cucurbita maxima | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no effect: Mg2+, Mn2+, Co2+ | Cucurbita maxima |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 84000 | - |
2 * 84000, SDS-PAGE | Cucurbita maxima |
| 160000 | - |
gel filtration | Cucurbita maxima |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine | Cucurbita maxima | biosynthesis of ethylene: plant hormone | 1-aminocyclopropane-1-carboxylate + methylthioadenosine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cucurbita maxima | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Cucurbita maxima |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fruit | - |
Cucurbita maxima | - |
| mesocarp | - |
Cucurbita maxima | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Cucurbita maxima |
| 0.22 | - |
- |
Cucurbita maxima |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | not: S-adenosylhomocysteine | Cucurbita maxima | ? | - |
? | |
| additional information | L-methionine | Cucurbita maxima | ? | - |
? | |
| additional information | S-methylthionine | Cucurbita maxima | ? | - |
? | |
| S-adenosyl-L-methionine | biosynthesis of ethylene: plant hormone | Cucurbita maxima | 1-aminocyclopropane-1-carboxylate + methylthioadenosine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 84000, SDS-PAGE | Cucurbita maxima |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
- |
Cucurbita maxima |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 10 | - |
Cucurbita maxima |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | small amounts, firmly bound | Cucurbita maxima | |
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