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Literature summary for 4.4.1.13 extracted from

  • Lodha, P.H.; Jaworski, A.F.; Aitken, S.M.
    Characterization of site-directed mutants of residues R58, R59, D116, W340 and R372 in the active site of E. coli cystathionine beta-lyase (2010), Protein Sci., 19, 383-391.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.4.1.13

Cloned(Commentary)

Cloned (Comment) Organism
mutant enzymes are expressed in the Escherichia coli KS1000 metC:cat strain Escherichia coli

Protein Variants

Protein Variants Comment Organism
D116A mutant with reduced catalytic efficiency Escherichia coli
D116N mutant with reduced catalytic efficiency Escherichia coli
R372A mutant with reduced catalytic efficiency Escherichia coli
R372K mutant with reduced catalytic efficiency Escherichia coli
R372L mutant with reduced catalytic efficiency Escherichia coli
R58A mutant with reduced catalytic efficiency Escherichia coli
R58K mutant with reduced catalytic efficiency Escherichia coli
R59A mutant with reduced catalytic efficiency Escherichia coli
R59K mutant with reduced catalytic efficiency Escherichia coli
W340F mutant with reduced catalytic efficiency Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
aminoethoxyvinylglycine
-
 Escherichia coli
N-hydrazinocarbonylmethyl-2-nitrobenzamide
-
 Escherichia coli
N-hydrazinocarbonylmethyl-2-trifluoromethylbenzamide
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
 L-cystathionine wild type enzyme, at 25°C Escherichia coli
0.55
-
 L-cystathionine mutant enzyme D116N, at 25°C Escherichia coli
0.67
-
 L-cystathionine mutant enzyme D116A, at 25°C Escherichia coli
1.02
-
 L-cystathionine mutant enzyme R59A, at 25°C Escherichia coli
1.35
-
 L-cystathionine mutant enzyme W340F, at 25°C Escherichia coli
1.51
-
 L-cystathionine mutant enzyme R59K, at 25°C Escherichia coli
5.1
-
 L-cystathionine mutant enzyme R58A, at 25°C Escherichia coli
15
-
 L-cystathionine mutant enzyme R372K, at 25°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-cystathionine + H2O
-
 Escherichia coli L-homocysteine + NH3 + pyruvate
-
 ?

Synonyms

Synonyms Comment Organism
CBL
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
8.1
-
 L-cystathionine mutant enzyme R372K, at 25°C Escherichia coli
9.7
-
 L-cystathionine mutant enzyme R58A, at 25°C Escherichia coli
34.1
-
 L-cystathionine wild type enzyme, at 25°C Escherichia coli
36.7
-
 L-cystathionine mutant enzyme R59K, at 25°C Escherichia coli
37.9
-
 L-cystathionine mutant enzyme D116N, at 25°C Escherichia coli
41.9
-
 L-cystathionine mutant enzyme D116A, at 25°C Escherichia coli
45.8
-
 L-cystathionine mutant enzyme R59A, at 25°C Escherichia coli
79
-
 L-cystathionine mutant enzyme W340F, at 25°C Escherichia coli

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0019
-
 aminoethoxyvinylglycine wild type enzyme, at 25°C Escherichia coli
0.0027
-
 aminoethoxyvinylglycine mutant enzyme D116N, at 25°C Escherichia coli
0.0027
-
 aminoethoxyvinylglycine mutant enzyme R59A, at 25°C Escherichia coli
0.0039
-
 aminoethoxyvinylglycine mutant enzyme D116A, at 25°C Escherichia coli
0.006
-
 aminoethoxyvinylglycine mutant enzyme R58A, at 25°C Escherichia coli
0.012
-
 aminoethoxyvinylglycine mutant enzyme R59K, at 25°C Escherichia coli
0.04
-
 aminoethoxyvinylglycine mutant enzyme W340F, at 25°C Escherichia coli
0.055
-
 aminoethoxyvinylglycine mutant enzyme R58K, at 25°C Escherichia coli
9.1
-
 aminoethoxyvinylglycine mutant enzyme R372K, at 25°C Escherichia coli

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00134
-
 mutant enzyme R59A, at 25°C Escherichia coli aminoethoxyvinylglycine
0.0017
-
 wild type enzyme, at 25°C Escherichia coli aminoethoxyvinylglycine
0.00174
-
 mutant enzyme R58A, at 25°C Escherichia coli aminoethoxyvinylglycine
0.0026
-
 mutant enzyme D116A, at 25°C Escherichia coli aminoethoxyvinylglycine
0.0033
-
 mutant enzyme D116N, at 25°C Escherichia coli aminoethoxyvinylglycine
0.0046
-
 mutant enzyme R59K, at 25°C Escherichia coli aminoethoxyvinylglycine
0.023
-
 mutant enzyme R58K, at 25°C Escherichia coli aminoethoxyvinylglycine
0.043
-
 mutant enzyme W340F, at 25°C Escherichia coli aminoethoxyvinylglycine
3.5
-
 mutant enzyme R372K, at 25°C Escherichia coli aminoethoxyvinylglycine

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.00009
-
 L-cystathionine mutant enzyme R372A, at 25°C Escherichia coli
0.00023
-
 L-cystathionine mutant enzyme R372L, at 25°C Escherichia coli
0.385
-
 L-cystathionine mutant enzyme R58K, at 25°C Escherichia coli
0.55
-
 L-cystathionine mutant enzyme R372K, at 25°C Escherichia coli
1.92
-
 L-cystathionine mutant enzyme R58A, at 25°C Escherichia coli
24
-
 L-cystathionine mutant enzyme R59K, at 25°C Escherichia coli
45
-
 L-cystathionine mutant enzyme R59A, at 25°C Escherichia coli
59
-
 L-cystathionine mutant enzyme W340F, at 25°C Escherichia coli
62
-
 L-cystathionine mutant enzyme D116A, at 25°C Escherichia coli
69
-
 L-cystathionine mutant enzyme D116N, at 25°C Escherichia coli
190
-
 L-cystathionine wild type enzyme, at 25°C Escherichia coli