Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.4.1.13 extracted from

  • Irmler, S.; Schäfer, H.; Beisert, B.; Rauhut, D.; Berthoud, H.
    Identification and characterization of a strain-dependent cystathionine beta/gamma-lyase in Lactobacillus casei potentially involved in cysteine biosynthesis (2009), FEMS Microbiol. Lett., 295, 67-76.
    View publication on PubMed
Search for more literature for 4.4.1.13

Activating Compound

Activating Compound Comment Organism Structure
NaCl NaCl considerably enhances the reaction rate at pH 6.8 Lacticaseibacillus casei

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Lacticaseibacillus casei

Inhibitors

Inhibitors Comment Organism Structure
NaCl in the presence of 4% (w/v) NaCl the reaction rate of Ctl1 is decreased to 40% of the original rate at pH 5.5; in the presence of 4% (w/v) NaCl the reaction rate of Ctl2 is decreased to 40% of the original rate at pH 5.5 Lacticaseibacillus casei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
 L-cysteine Ctl2, at pH 5.5 and 37°C Lacticaseibacillus casei
0.33
-
 L-cystathionine Ctl2, in sodium phosphate buffer, at pH 5.5 and 37°C Lacticaseibacillus casei
0.35
-
 L-cystathionine Ctl1, in sodium phosphate buffer, at pH 5.5 and 37°C Lacticaseibacillus casei
0.37
-
 L-cystathionine Ctl1, in sodium phosphate buffer, at pH 6.8 and 37°C Lacticaseibacillus casei
0.41
-
 L-cystathionine Ctl2, in sodium phosphate buffer, at pH 6.8 and 37°C Lacticaseibacillus casei
1
-
 L-cysteine Ctl1, at pH 5.5 and 37°C Lacticaseibacillus casei
1
-
 DL-homocysteine Ctl2, at pH 5.5 and 37°C Lacticaseibacillus casei
1.4
-
 DL-homocysteine Ctl1, at pH 5.5 and 37°C Lacticaseibacillus casei
32.8
-
 L-methionine Ctl1, at pH 5.5 and 37°C Lacticaseibacillus casei
34.2
-
 L-methionine Ctl2, at pH 5.5 and 37°C Lacticaseibacillus casei

Organism

Organism UniProt Comment Textmining
Lacticaseibacillus casei B3TNN8 cystathionine beta- and gamma-lyase Ctl1; strain ATCC 334
-
Lacticaseibacillus casei B3TNN9 cystathionine beta- and gamma-lyase Ctl2; strain ATCC 334
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+ affinity column chromatography Lacticaseibacillus casei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DL-homocysteine + H2O Ctl1 degrades DL-homocysteine at pH 5.5, while no activity is detected at pH 6.8 or 9.0 Lacticaseibacillus casei hydrogen sulfide + 2-oxobutyrate + ?
-
 ?
DL-homocysteine + H2O Ctl2 degrades DL-homocysteine at pH 5.5, while no activity is detected at pH 6.8 or 9.0 Lacticaseibacillus casei hydrogen sulfide + 2-oxobutyrate + ?
-
 ?
L-cystathionine + H2O recombinant Ctl1 shows high activity toward the degradation of L-cystathionine Lacticaseibacillus casei L-homocysteine + NH3 + pyruvate
-
 ?
L-cystathionine + H2O recombinant Ctl2 shows high activity toward the degradation of L-cystathionine Lacticaseibacillus casei L-homocysteine + NH3 + pyruvate
-
 ?
L-cysteine + H2O Ctl1 degrades L-cysteine at pH 5.5, while no activity is detected at pH 6.8 or 9.0 Lacticaseibacillus casei hydrogen sulfide + pyruvate + ?
-
 ?
L-cysteine + H2O Ctl2 degrades L-cysteine at pH 5.5, while no activity is detected at pH 6.8 or 9.0 Lacticaseibacillus casei hydrogen sulfide + pyruvate + ?
-
 ?
L-methionine + H2O + ? Ctl1 degrades L-methionine at pH 5.5, while no activity is detected at pH 6.8 or 9.0 Lacticaseibacillus casei methanethiol + dimethyl disulfide + dimethyl trisulfide + 2-oxobutyrate + ?
-
 ?
L-methionine + H2O + ? Ctl2 degrades L-methionine at pH 5.5, while no activity is detected at pH 6.8 or 9.0 Lacticaseibacillus casei methanethiol + dimethyl disulfide + dimethyl trisulfide + 2-oxobutyrate + ?
-
 ?

Synonyms

Synonyms Comment Organism
CBL
-
 Lacticaseibacillus casei
Ctl1
-
 Lacticaseibacillus casei
Ctl2
-
 Lacticaseibacillus casei
cystathionine beta/gamma-lyase
-
 Lacticaseibacillus casei

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
42 57 Ctl1 does not lose activity after incubation at 42°C for 30 min, but activity is lost at 57°C Lacticaseibacillus casei
42 57 Ctl2 does not lose activity after incubation at 42°C for 30 min, but activity is lost at 57°C Lacticaseibacillus casei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Lacticaseibacillus casei

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.8 9 at pH 6.8 Ctl1 still exhibits high cystathionine lyase activity, no cystathionine lyase activity is detected at pH 9.0 Lacticaseibacillus casei
6.8 9 at pH 6.8 cystathionine lyase activity of Ctl2 is strongly reduced, no cystathionine lyase activity is detected at pH 9.0 Lacticaseibacillus casei

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Lacticaseibacillus casei