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Literature summary for 4.4.1.13 extracted from
- Cloning and characterization of two Lactobacillus casei genes encoding a cystathionine lyase (2008), Appl. Environ. Microbiol., 74, 99-106.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, expression of His-tagged MalY in Escherichia coli | Lacticaseibacillus casei |
| DNA and amino acid sequence determination and analysis, expression of His-tagged MetC in Escherichia coli | Lacticaseibacillus casei |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no inhibition of MalY by NaCl | Lacticaseibacillus casei | |
| NaCl | complete inhibition of MetC at 4% | Lacticaseibacillus casei |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
x * 43000, recombinant His-tagged MetC, SDS-PAGE | Lacticaseibacillus casei |
| 47000 | - |
x * 47000, recombinant His-tagged MalY, SDS-PAGE | Lacticaseibacillus casei |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-cystathionine + H2O | Lacticaseibacillus casei | - |
L-homocysteine + NH3 + pyruvate | - |
? | |
| L-cysteine + H2O | Lacticaseibacillus casei | - |
sulfide + NH3 + pyruvate | - |
? | |
| L-methionine + H2O | Lacticaseibacillus casei | - |
methanethiol + NH3 + 2-oxobutanoate | - |
? | |
| additional information | Lacticaseibacillus casei | the enzyme is implicated in the degradation of not only cystathionine but also cysteine and methionine | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lacticaseibacillus casei | - |
strain ATCC 334 and several other genotypes, overview, gene metC | - |
| Lacticaseibacillus casei | A6YH85 | gene malY; strain ATCC 334 and several other genotypes, overview, gene malY | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged MalY from Escherichia coli by nickel affinity chromatography | Lacticaseibacillus casei |
| recombinant His-tagged MetC from Escherichia coli by nickel affinity chromatography | Lacticaseibacillus casei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-cystathionine + H2O | - |
Lacticaseibacillus casei | L-homocysteine + NH3 + pyruvate | - |
? | |
| L-cysteine + H2O | - |
Lacticaseibacillus casei | sulfide + NH3 + pyruvate | - |
? | |
| L-cysteine + H2O | MalY | Lacticaseibacillus casei | sulfide + NH3 + pyruvate | - |
? | |
| L-methionine + H2O | - |
Lacticaseibacillus casei | methanethiol + NH3 + 2-oxobutanoate | - |
? | |
| L-serine + H2O | MalY | Lacticaseibacillus casei | hydrogen sulfide + pyruvate + NH3 | - |
? | |
| additional information | the enzyme is implicated in the degradation of not only cystathionine but also cysteine and methionine | Lacticaseibacillus casei | ? | - |
? | |
| additional information | the enzyme performs a beta-elimination reaction, substrate specificity and product determination, overview | Lacticaseibacillus casei | ? | - |
? | |
| O-succinyl-L-homoserine + H2O | MetC | Lacticaseibacillus casei | 2-oxobutyrate + succinate + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 43000, recombinant His-tagged MetC, SDS-PAGE | Lacticaseibacillus casei |
| ? | x * 47000, recombinant His-tagged MalY, SDS-PAGE | Lacticaseibacillus casei |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CBL | - |
Lacticaseibacillus casei |
| cystathionine lyase | - |
Lacticaseibacillus casei |
| MalY | - |
Lacticaseibacillus casei |
| MetC | - |
Lacticaseibacillus casei |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Lacticaseibacillus casei |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 56 | - |
30 min, inactivation | Lacticaseibacillus casei |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
MalY | Lacticaseibacillus casei |
| 9 | - |
MetC | Lacticaseibacillus casei |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9 | MetC: 15% of maximal activity at pH 5.5, optimal activity at pH 9.0 | Lacticaseibacillus casei |
| 5.5 | 6.8 | MalY: 55% of maximal activity at pH 5.5, optimal activity at pH 6.0, reduced activity at pH 6.8, no activity at pH 9.0 | Lacticaseibacillus casei |
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