Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DL-homocysteine | 12.5 mM, 70% inhibition, competitive inhibitor | Brassica oleracea | |
hydroxylamine | 12.5 mM, 97% inhibition | Brassica oleracea | |
L-cysteine | 12 mM, 65% inhibition after 5 min, 1 mM, 29% inhibition after 10 min, linear noncompetitive inhibitor | Brassica oleracea | |
NEM | 1.25 mM, about 45% inhibition | Brassica oleracea | |
pyridoxal | 12.5 mM, in presence of 12 mM L-cystine 12% inhibition, in presence of 1 mM L-cystine 37% inhibition | Brassica oleracea | |
pyruvate | 12.5 mM, 25% inhibition | Brassica oleracea | |
Sodium cyanide | 12.5 mM, 95% inhibition | Brassica oleracea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0812 | - |
L-cystine | - |
Brassica oleracea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
2 * 48000, SDS-PAGE | Brassica oleracea |
100000 | - |
gel filtration | Brassica oleracea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Brassica oleracea | the enzyme is responsible for off-aroma deterioration in fresh unblanched broccoli | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brassica oleracea | - |
var. italica | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | carbohydrate content of 3% | Brassica oleracea |
Purification (Comment) | Organism |
---|---|
- |
Brassica oleracea |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.69 | - |
- |
Brassica oleracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cystathionine + H2O | 1-4% of the activity with L-cysteine, depending on assay method | Brassica oleracea | L-homocysteine + NH3 + pyruvate | - |
? | |
D-cystine + H2O | 2% of the activity with L-cystine | Brassica oleracea | ? | - |
? | |
L-cysteine S-sulfate + H2O | 11% of the activity with L-cystine | Brassica oleracea | ? | - |
? | |
L-cystine + H2O | - |
Brassica oleracea | L-thiocysteine + pyruvate + NH3 | - |
? | |
L-djenkolic acid + H2O | 18-24% of the activity with L-cystine, depending on assay method | Brassica oleracea | ? | - |
? | |
additional information | the enzyme is responsible for off-aroma deterioration in fresh unblanched broccoli | Brassica oleracea | ? | - |
? | |
S-ethyl-L-cysteine + H2O | 18% of the activity with L-cystine | Brassica oleracea | ? | - |
? | |
S-ethyl-L-cysteine sulfoxide + H2O | 71% of the activity with L-cystine | Brassica oleracea | ? | - |
? | |
S-methyl-L-cysteine + H2O | 13-29% of the activity with L-cystine, depending on assay method | Brassica oleracea | ? | - |
? | |
S-propyl-L-cysteine + H2O | 29% of the activity with L-cystine | Brassica oleracea | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 48000, SDS-PAGE | Brassica oleracea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Brassica oleracea |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
10 min, completely stable up to | Brassica oleracea |
50 | - |
10 min, 20% loss of activity | Brassica oleracea |
60 | - |
10 min, 75% loss of actrivity | Brassica oleracea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.1 | - |
- |
Brassica oleracea |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8.4 | 9.9 | pH 8.4: about 55% of maximal activity, pH 9.9: about 30% of maximal activity | Brassica oleracea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | contains 2 mol per mol of enzyme | Brassica oleracea |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
1.5 | - |
DL-homocysteine | - |
Brassica oleracea | |
5 | - |
L-cysteine | - |
Brassica oleracea |