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Literature summary for 4.4.1.13 extracted from

  • Dwivedi, C.M.; Ragin, R.C.; Uren, J.R.
    Cloning, purification, and characterization of beta-cystathionase from Escherichia coli (1982), Biochemistry, 21, 3064-3069.
    View publication on PubMed
Search for more literature for 4.4.1.13

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli

General Stability

General Stability Organism
not stable to lyophilization Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
3,3,3,-trifluoroalanine irreversible Escherichia coli
Beta-cyanoalanine
-
 Escherichia coli
Cys competitive with L-cystathionine Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 L-cystathionine
-
 Escherichia coli
0.25
-
 L-cystine
-
 Escherichia coli
0.36
-
 L-djenkolate
-
 Escherichia coli
0.83
-
 L-meso-lanthionine
-
 Escherichia coli
4.5
-
 L-homolanthionine
-
 Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
280000
-
 gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
215
-
-
 Escherichia coli

Storage Stability

Storage Stability Organism
-20°C or 4°C, 50% glycerol, 4 months, stable Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-cystathionine + H2O
-
 Escherichia coli L-homocysteine + NH3 + pyruvate
-
 ?
L-cysteine + H2O
-
 Escherichia coli ?
-
 ?
L-cystine + H2O
-
 Escherichia coli L-thiocysteine + pyruvate + NH3
-
 ?
L-djenkolate + H2O
-
 Escherichia coli pyruvate
-
 ?
L-homolanthionine + H2O
-
 Escherichia coli ?
-
 ?
L-meso-lanthionine + H2O
-
 Escherichia coli ?
-
 ?

Subunits

Subunits Comment Organism
hexamer
-
 Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9
-
 Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
7.2 9 pH 7.2: 36% of maximal activity, pH 8.0-9.0: optimum Escherichia coli

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate 5.7 mol of pyridoxal 5'-phosphate is bound per mol of enzyme Escherichia coli
pyridoxal 5'-phosphate Km: 0.005 mM Escherichia coli