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Literature summary for 4.4.1.13 extracted from

  • Alting, A.C.; Engels, W.J.M.; van Schalkwijk, S.; Exterkate, F.A.
    Purification and characterization of cystathionine beta-lyase from Lactococcus lactis subsp. cremonis B78 and its possible role in flavor development in cheese (1995), Appl. Environ. Microbiol., 61, 4037-4042.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
nutrition possible essential role of the enzyme in flavor development in cheese is suggested Lactococcus lactis

General Stability

General Stability Organism
repeated freezing, -20°C, and thawing has no effect Lactococcus lactis

Inhibitors

Inhibitors Comment Organism Structure
3-methyl-2-benzothiazolinone hydrazoone
-
Lactococcus lactis
Beta-cyanoalanine competitive; strong Lactococcus lactis
carboxymethoxylamine irreversible Lactococcus lactis
DL-cycloserine irreversible Lactococcus lactis
DL-Penicillamine
-
Lactococcus lactis
DL-propargylglycine irreversible Lactococcus lactis
hydroxylamine
-
Lactococcus lactis
phenylhydrazine
-
Lactococcus lactis
Semicarbazide
-
Lactococcus lactis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
130000
-
non-denaturing PAGE Lactococcus lactis
165000
-
gel filtration Lactococcus lactis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cystathionine + H2O Lactococcus lactis enzyme is involved in methionine biosynthesis ?
-
?

Organism

Organism UniProt Comment Textmining
Lactococcus lactis
-
subsp. cremonis B78
-

Purification (Commentary)

Purification (Comment) Organism
-
Lactococcus lactis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1
-
-
Lactococcus lactis

Storage Stability

Storage Stability Organism
-20°C, stable for months Lactococcus lactis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cystathionine + H2O enzyme is involved in methionine biosynthesis Lactococcus lactis ?
-
?
homoserine + H2O 96% of the activity with L-cystathionine Lactococcus lactis ?
-
?
L-cystathionine + H2O alpha,beta-elimination Lactococcus lactis L-homocysteine + NH3 + pyruvate
-
?
L-cystathionine + H2O alpha,gamma-elimination Lactococcus lactis cysteine + ?
-
?
L-cysteine + H2O 12% of the activity with L-cystathionine Lactococcus lactis ?
-
?
L-cystine + H2O 64% of the activity with L-cystathionine Lactococcus lactis L-thiocysteine + pyruvate + NH3
-
?
L-djenkolate + H2O 58% of the activity with L-cystathionine Lactococcus lactis pyruvate
-
?
L-homocysteine + H2O 4% of the activity with L-cystathionine Lactococcus lactis ?
-
?
lanthionine + H2O 169% of the activity with L-cystathionine Lactococcus lactis L-cysteine + NH3 + pyruvate
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 35000-40000, SDS-PAGE Lactococcus lactis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
20 h, stable Lactococcus lactis
20
-
20 h, stable Lactococcus lactis
60
-
10 min, stable up to Lactococcus lactis
70
-
10 min, complete inactivation above Lactococcus lactis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.5 rapid decrease of activity above pH 8.5 Lactococcus lactis

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate dependent on Lactococcus lactis