Inhibitors | Comment | Organism | Structure |
---|---|---|---|
HgCl2 | 57% inhibition at 1 mM | Bacillus sp. (in: Bacteria) | |
hydoxylamine | complete inhibition at 1 mM | Bacillus sp. (in: Bacteria) | |
p-chloromercuribenzoate | 82% inhibition at 1 mM | Bacillus sp. (in: Bacteria) | |
phenylhydrazine | complete inhibition at 1 mM | Bacillus sp. (in: Bacteria) |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.73 | - |
S-Benzyl-L-cysteine | - |
Bacillus sp. (in: Bacteria) | |
2 | - |
L-selenodjenkolate | - |
Bacillus sp. (in: Bacteria) | |
3.6 | - |
L-djenkolate | - |
Bacillus sp. (in: Bacteria) | |
4.6 | - |
Se-(methyl)-L-selenocysteine | - |
Bacillus sp. (in: Bacteria) | |
4.8 | - |
L-cysteine | - |
Bacillus sp. (in: Bacteria) | |
5.1 | - |
S-methyl-L-cysteine | - |
Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
2 * 39000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
76000 | - |
gel filtration | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
41A | - |
Bacillus sp. (in: Bacteria) 41A | - |
41A | - |
Purification (Comment) | Organism |
---|---|
- |
Bacillus sp. (in: Bacteria) |
Storage Stability | Organism |
---|---|
-20°C, 10 mM potassium phospate buffer, pH 7.2, with 50% glycerol, 0.01 mM pyridoxal 5'-phosphate and 0.01% 2-mercaptoethanol, 1 month without loss of activity | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-cysteine + H2O | - |
Bacillus sp. (in: Bacteria) | pyruvate + NH3 + hydrogen sulfide | - |
? | |
L-cysteine + H2O | - |
Bacillus sp. (in: Bacteria) 41A | pyruvate + NH3 + hydrogen sulfide | - |
? | |
L-djenkolate | - |
Bacillus sp. (in: Bacteria) | pyruvate + NH3 + S-(mercaptomethyl)cysteine | - |
? | |
L-djenkolate | - |
Bacillus sp. (in: Bacteria) 41A | pyruvate + NH3 + S-(mercaptomethyl)cysteine | - |
? | |
L-Selenodjenkolate | - |
Bacillus sp. (in: Bacteria) | Se-(hydroselenomethyl)selenocysteine + pyruvate + NH3 | - |
? | |
L-Selenodjenkolate | - |
Bacillus sp. (in: Bacteria) 41A | Se-(hydroselenomethyl)selenocysteine + pyruvate + NH3 | - |
? | |
S-benzyl-L-cysteine + H2O | - |
Bacillus sp. (in: Bacteria) | ? | - |
? | |
S-benzyl-L-cysteine + H2O | - |
Bacillus sp. (in: Bacteria) 41A | ? | - |
? | |
S-methyl-L-cysteine | - |
Bacillus sp. (in: Bacteria) | methyl mercaptane + pyruvate + NH3 | - |
? | |
S-methyl-L-cysteine | - |
Bacillus sp. (in: Bacteria) 41A | methyl mercaptane + pyruvate + NH3 | - |
? | |
Se-(methyl)-L-selenocysteine | - |
Bacillus sp. (in: Bacteria) | HSe(CH3) + pyruvate + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 39000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
- |
Bacillus sp. (in: Bacteria) |