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Literature summary for 4.4.1.11 extracted from

  • Morozova, E.; Kulikova, V.; Rodionov, A.; Revtovich, S.; Anufrieva, N.; Demidkina, T.
    Engineered Citrobacter freundii methionine gamma-lyase effectively produces antimicrobial thiosulfinates (2016), Biochimie, 128-129, 92-98 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Citrobacter freundii

Protein Variants

Protein Variants Comment Organism
C115A mutation leads to a decrease of the catalytic efficiency in the gamma-elimination reaction of the physiological substrate by more than an order of magnitude Citrobacter freundii
C115H mutation leads to loss of activity in the gamma-elimination reaction of the physiological substrate. The catalytic efficiency in the beta-elimination reaction of S-substituted L-cysteine sulfoxides is increased by about an order of magnitude compared to the wild type Citrobacter freundii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.26
-
S-ethyl-L-cysteine mutant C115A, pH 8.0, 30°C Citrobacter freundii
0.3
-
L-methionine mutant C115A, pH 8.0, 30°C Citrobacter freundii
0.44
-
alliin mutant C115H, pH 8.0, 30°C Citrobacter freundii
0.457
-
ethiin mutant C115H, pH 8.0, 30°C Citrobacter freundii
0.57
-
methiin mutant C115H, pH 8.0, 30°C Citrobacter freundii
0.63
-
methiin mutant C115A, pH 8.0, 30°C Citrobacter freundii
0.8
-
S-methyl-L-cysteine mutant C115A, pH 8.0, 30°C Citrobacter freundii
0.93
-
S-ethyl-L-cysteine mutant C115H, pH 8.0, 30°C Citrobacter freundii
1.75
-
alliin mutant C115A, pH 8.0, 30°C Citrobacter freundii
2.46
-
O-acetyl-L-homoserine mutant C115H, pH 8.0, 30°C Citrobacter freundii
2.62
-
S-methyl-L-cysteine mutant C115H, pH 8.0, 30°C Citrobacter freundii
2.88
-
O-acetyl-L-serine mutant C115A, pH 8.0, 30°C Citrobacter freundii
3.68
-
O-acetyl-L-serine mutant C115H, pH 8.0, 30°C Citrobacter freundii
5.48
-
ethiin mutant C115A, pH 8.0, 30°C Citrobacter freundii
6
-
O-acetyl-L-homoserine mutant C115A, pH 8.0, 30°C Citrobacter freundii

Organism

Organism UniProt Comment Textmining
Citrobacter freundii Q84AR1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alliin + H2O
-
Citrobacter freundii allicin + pyruvic acid + NH3
-
?
ethiin + H2O
-
Citrobacter freundii ethanesulfenic acid + pyruvate + NH3
-
?
L-methionine + H2O
-
Citrobacter freundii methanethiol + NH3 + 2-oxobutanoate
-
?
methiin + H2O
-
Citrobacter freundii methanesulfenic acid + pyruvate + NH3
-
?
O-acetyl-L-homoserine + H2O
-
Citrobacter freundii 2-oxobutanoate + NH3 + acetate
-
?
O-acetyl-L-serine + H2O
-
Citrobacter freundii 2-oxopropanoate + NH3 + acetate
-
?
S-ethyl-L-cysteine + H2O
-
Citrobacter freundii ethanethiol + NH3 + 2-oxopropanoate
-
?
S-methyl-L-cysteine + H2O
-
Citrobacter freundii methanethiol + NH3 + 2-oxopropanoate
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.22
-
L-methionine mutant C115A, pH 8.0, 30°C Citrobacter freundii
0.41
-
alliin mutant C115A, pH 8.0, 30°C Citrobacter freundii
0.57
-
S-ethyl-L-cysteine mutant C115A, pH 8.0, 30°C Citrobacter freundii
1.25
-
ethiin mutant C115A, pH 8.0, 30°C Citrobacter freundii
1.35
-
O-acetyl-L-serine mutant C115A, pH 8.0, 30°C Citrobacter freundii
1.38
-
S-methyl-L-cysteine mutant C115A, pH 8.0, 30°C Citrobacter freundii
1.63
-
methiin mutant C115A, pH 8.0, 30°C Citrobacter freundii
2.93
-
O-acetyl-L-serine mutant C115H, pH 8.0, 30°C Citrobacter freundii
5.14
-
methiin mutant C115H, pH 8.0, 30°C Citrobacter freundii
5.59
-
ethiin mutant C115H, pH 8.0, 30°C Citrobacter freundii
6.23
-
O-acetyl-L-homoserine mutant C115A, pH 8.0, 30°C Citrobacter freundii
6.5
-
S-ethyl-L-cysteine mutant C115H, pH 8.0, 30°C Citrobacter freundii
11.31
-
alliin mutant C115H, pH 8.0, 30°C Citrobacter freundii
18.29
-
O-acetyl-L-homoserine mutant C115H, pH 8.0, 30°C Citrobacter freundii
23.08
-
S-methyl-L-cysteine mutant C115H, pH 8.0, 30°C Citrobacter freundii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.23
-
alliin mutant C115A, pH 8.0, 30°C Citrobacter freundii
0.23
-
ethiin mutant C115A, pH 8.0, 30°C Citrobacter freundii
0.46
-
O-acetyl-L-serine mutant C115A, pH 8.0, 30°C Citrobacter freundii
0.69
-
L-methionine mutant C115A, pH 8.0, 30°C Citrobacter freundii
0.8
-
O-acetyl-L-serine mutant C115H, pH 8.0, 30°C Citrobacter freundii
1.04
-
O-acetyl-L-homoserine mutant C115A, pH 8.0, 30°C Citrobacter freundii
1.7
-
S-methyl-L-cysteine mutant C115A, pH 8.0, 30°C Citrobacter freundii
2.24
-
S-ethyl-L-cysteine mutant C115A, pH 8.0, 30°C Citrobacter freundii
2.6
-
methiin mutant C115A, pH 8.0, 30°C Citrobacter freundii
6.99
-
S-ethyl-L-cysteine mutant C115H, pH 8.0, 30°C Citrobacter freundii
7.43
-
O-acetyl-L-homoserine mutant C115H, pH 8.0, 30°C Citrobacter freundii
8.81
-
S-methyl-L-cysteine mutant C115H, pH 8.0, 30°C Citrobacter freundii
9.02
-
methiin mutant C115H, pH 8.0, 30°C Citrobacter freundii
12
-
ethiin mutant C115H, pH 8.0, 30°C Citrobacter freundii
25.7
-
alliin mutant C115H, pH 8.0, 30°C Citrobacter freundii