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Literature summary for 4.4.1.11 extracted from

  • Anufrieva, N.V.; Faleev, N.G.; Morozova, E.A.; Bazhulina, N.P.; Revtovich, S.V.; Timofeev, V.P.; Tkachev, Y.V.; Nikulin, A.D.; Demidkina, T.V.
    The role of active site tyrosine 58 in Citrobacter freundii methionine gamma-lyase (2015), Biochim. Biophys. Acta, 1854, 1220-1228 .
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
structure of mutant Y58F, to 1.96 A resolution. The mutation does not result in essential changes of the conformation of the active site Citrobacter freundii

Protein Variants

Protein Variants Comment Organism
Y58F mutation leads to a decrease of catalytic efficiencies in both gamma- and beta-elimination reactions of about two orders of magnitude and to a change of rate the limiting step of the physiological reaction Citrobacter freundii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.75
-
S-Benzyl-L-cysteine mutant Y58F, pH 8.0, 30°C Citrobacter freundii
3.46
-
DL-homocysteine mutant Y58F, pH 8.0, 30°C Citrobacter freundii
8.11
-
S-ethyl-L-homocysteine mutant Y58F, pH 8.0, 30°C Citrobacter freundii
8.19
-
S-ethyl-L-cysteine mutant Y58F, pH 8.0, 30°C Citrobacter freundii
19.65
-
L-methionine mutant Y58F, pH 8.0, 30°C Citrobacter freundii

Organism

Organism UniProt Comment Textmining
Citrobacter freundii Q84AR1
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.25
-
mutant Y58F, pH 8.0, 30°C Citrobacter freundii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DL-homocysteine + H2O
-
Citrobacter freundii 2-oxobutanoate + NH3 + hydrogen sulfide
-
?
L-methionine + H2O
-
Citrobacter freundii methanethiol + NH3 + 2-oxobutanoate
-
?
S-benzyl-L-cysteine + H2O
-
Citrobacter freundii thiobenzyl alcohol + NH3 + 2-oxopropanoate
-
?
S-ethyl-L-cysteine + H2O
-
Citrobacter freundii ethanethiol + NH3 + 2-oxopropanoate
-
?
S-ethyl-L-homocysteine + H2O
-
Citrobacter freundii ethanethiol + NH3 + 2-oxobutanoate
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.88
-
DL-homocysteine mutant Y58F, pH 8.0, 30°C Citrobacter freundii
2.07
-
S-ethyl-L-homocysteine mutant Y58F, pH 8.0, 30°C Citrobacter freundii
2.38
-
S-ethyl-L-cysteine mutant Y58F, pH 8.0, 30°C Citrobacter freundii
2.66
-
L-methionine mutant Y58F, pH 8.0, 30°C Citrobacter freundii
3.34
-
S-Benzyl-L-cysteine mutant Y58F, pH 8.0, 30°C Citrobacter freundii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.121
-
S-Benzyl-L-cysteine mutant Y58F, pH 8.0, 30°C Citrobacter freundii
0.135
-
L-methionine mutant Y58F, pH 8.0, 30°C Citrobacter freundii
0.254
-
DL-homocysteine mutant Y58F, pH 8.0, 30°C Citrobacter freundii
0.255
-
S-ethyl-L-homocysteine mutant Y58F, pH 8.0, 30°C Citrobacter freundii
0.291
-
S-ethyl-L-cysteine mutant Y58F, pH 8.0, 30°C Citrobacter freundii