Crystallization (Comment) | Organism |
---|---|
structure of mutant Y58F, to 1.96 A resolution. The mutation does not result in essential changes of the conformation of the active site | Citrobacter freundii |
Protein Variants | Comment | Organism |
---|---|---|
Y58F | mutation leads to a decrease of catalytic efficiencies in both gamma- and beta-elimination reactions of about two orders of magnitude and to a change of rate the limiting step of the physiological reaction | Citrobacter freundii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.75 | - |
S-Benzyl-L-cysteine | mutant Y58F, pH 8.0, 30°C | Citrobacter freundii | |
3.46 | - |
DL-homocysteine | mutant Y58F, pH 8.0, 30°C | Citrobacter freundii | |
8.11 | - |
S-ethyl-L-homocysteine | mutant Y58F, pH 8.0, 30°C | Citrobacter freundii | |
8.19 | - |
S-ethyl-L-cysteine | mutant Y58F, pH 8.0, 30°C | Citrobacter freundii | |
19.65 | - |
L-methionine | mutant Y58F, pH 8.0, 30°C | Citrobacter freundii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Citrobacter freundii | Q84AR1 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.25 | - |
mutant Y58F, pH 8.0, 30°C | Citrobacter freundii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DL-homocysteine + H2O | - |
Citrobacter freundii | 2-oxobutanoate + NH3 + hydrogen sulfide | - |
? | |
L-methionine + H2O | - |
Citrobacter freundii | methanethiol + NH3 + 2-oxobutanoate | - |
? | |
S-benzyl-L-cysteine + H2O | - |
Citrobacter freundii | thiobenzyl alcohol + NH3 + 2-oxopropanoate | - |
? | |
S-ethyl-L-cysteine + H2O | - |
Citrobacter freundii | ethanethiol + NH3 + 2-oxopropanoate | - |
? | |
S-ethyl-L-homocysteine + H2O | - |
Citrobacter freundii | ethanethiol + NH3 + 2-oxobutanoate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.88 | - |
DL-homocysteine | mutant Y58F, pH 8.0, 30°C | Citrobacter freundii | |
2.07 | - |
S-ethyl-L-homocysteine | mutant Y58F, pH 8.0, 30°C | Citrobacter freundii | |
2.38 | - |
S-ethyl-L-cysteine | mutant Y58F, pH 8.0, 30°C | Citrobacter freundii | |
2.66 | - |
L-methionine | mutant Y58F, pH 8.0, 30°C | Citrobacter freundii | |
3.34 | - |
S-Benzyl-L-cysteine | mutant Y58F, pH 8.0, 30°C | Citrobacter freundii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.121 | - |
S-Benzyl-L-cysteine | mutant Y58F, pH 8.0, 30°C | Citrobacter freundii | |
0.135 | - |
L-methionine | mutant Y58F, pH 8.0, 30°C | Citrobacter freundii | |
0.254 | - |
DL-homocysteine | mutant Y58F, pH 8.0, 30°C | Citrobacter freundii | |
0.255 | - |
S-ethyl-L-homocysteine | mutant Y58F, pH 8.0, 30°C | Citrobacter freundii | |
0.291 | - |
S-ethyl-L-cysteine | mutant Y58F, pH 8.0, 30°C | Citrobacter freundii |