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Literature summary for 4.4.1.11 extracted from
- Assays for methionine beta-lyase and S-adenosyl-L-homocysteine hydrolase based on enzymatic formation of CdS quantum dots in situ (2012), Anal. Chem., 84, 8961-8965.
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | the enzyme can be used for detection of S-adenosyl-L-homocysteine hydrolase activity and inhibition in a simple and robust fluorogenic enzymatic assay, method development, overview | Pseudomonas putida |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
4 * 43000, SDS-PAGE | Pseudomonas putida |
| 172000 | - |
gel filtration | Pseudomonas putida |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-homocysteine | Pseudomonas putida | - |
2-oxobutanoate + NH3 + hydrogen sulfide | - |
? |  |
| L-homocysteine | Pseudomonas putida ICR 3460 | - |
2-oxobutanoate + NH3 + hydrogen sulfide | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
- |
- |
| Pseudomonas putida ICR 3460 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme by anion exchange chromatography and gel filtration | Pseudomonas putida |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | - |
Pseudomonas putida | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-homocysteine | - |
Pseudomonas putida | 2-oxobutanoate + NH3 + hydrogen sulfide | - |
? | |
| L-homocysteine | - |
Pseudomonas putida | 2-oxobutanoate + NH3 + hydrogen sulfide | methionine gamma-lyase catalyzes the decomposition of homocysteine to hydrogen sulfide which forms fluorescent CdS nanoparticles in the presence of Cd(NO3)2. Hydrogen sulfide dissociates to yield S2? anions which readily interact with Cd2+ forming fluorescent CdS nanocrystals, assay method development and evaluation, overview | ? | |
| L-homocysteine | - |
Pseudomonas putida ICR 3460 | 2-oxobutanoate + NH3 + hydrogen sulfide | - |
? | |
| L-homocysteine | - |
Pseudomonas putida ICR 3460 | 2-oxobutanoate + NH3 + hydrogen sulfide | methionine gamma-lyase catalyzes the decomposition of homocysteine to hydrogen sulfide which forms fluorescent CdS nanoparticles in the presence of Cd(NO3)2. Hydrogen sulfide dissociates to yield S2? anions which readily interact with Cd2+ forming fluorescent CdS nanocrystals, assay method development and evaluation, overview | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 43000, SDS-PAGE | Pseudomonas putida |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MGL | - |
Pseudomonas putida |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Pseudomonas putida |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.4 | - |
assay at | Pseudomonas putida |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | 4 mol of pyridoxal 5'-phosphate per mole of enzyme | Pseudomonas putida | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the enzyme is responsible for the alpha,beta,gamma-elimination and gamma,beta-replacement in analogues of L-methionione such as L-homocysteine, through degradation to keto acids, ammonia, and H2S in anaerobic bacteria and parasitic protozoa | Pseudomonas putida |
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