Application | Comment | Organism |
---|---|---|
medicine | enzyme therapy against various types of methionine-dependent tumors | Entamoeba histolytica |
medicine | enzyme therapy against various types of methionine-dependent tumors | Lactococcus lactis |
medicine | enzyme therapy against various types of methionine-dependent tumors | Pseudomonas putida |
medicine | enzyme therapy against various types of methionine-dependent tumors | Trichomonas vaginalis |
medicine | enzyme therapy against various types of methionine-dependent tumors | Citrobacter freundii |
medicine | enzyme therapy against various types of methionine-dependent tumors | Citrobacter intermedius |
medicine | enzyme therapy against various types of methionine-dependent tumors | Clostridium sporogenes |
medicine | enzyme therapy against various types of methionine-dependent tumors | Aeromonas sp. |
medicine | enzyme therapy against various types of methionine-dependent tumors | Aspergillus sp. |
medicine | enzyme therapy against various types of methionine-dependent tumors | Treponema denticola |
medicine | enzyme therapy against various types of methionine-dependent tumors | Cladosporium cladosporioides |
medicine | enzyme therapy against various types of methionine-dependent tumors | Aspergillus flavipes |
medicine | enzyme therapy against various types of methionine-dependent tumors | Achromobacter starkeyi |
medicine | enzyme therapy against various types of methionine-dependent tumors | Brevibacterium linens BL2 |
Crystallization (Comment) | Organism |
---|---|
hanging-drop vapor-diffusion method | Entamoeba histolytica |
hanging-drop vapor-diffusion method | Lactococcus lactis |
hanging-drop vapor-diffusion method | Pseudomonas putida |
hanging-drop vapor-diffusion method | Trichomonas vaginalis |
hanging-drop vapor-diffusion method | Citrobacter freundii |
hanging-drop vapor-diffusion method | Citrobacter intermedius |
hanging-drop vapor-diffusion method | Clostridium sporogenes |
hanging-drop vapor-diffusion method | Aeromonas sp. |
hanging-drop vapor-diffusion method | Aspergillus sp. |
hanging-drop vapor-diffusion method | Treponema denticola |
hanging-drop vapor-diffusion method | Cladosporium cladosporioides |
hanging-drop vapor-diffusion method | Aspergillus flavipes |
hanging-drop vapor-diffusion method | Brevibacterium linens BL2 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Aspergillus flavipes | |
2-mercaptoethanol | - |
Lactococcus lactis | |
3-Methyl-2-benzothiazolinone hydrazone | - |
Lactococcus lactis | |
5-5'-dithiobis-(2-nitrobenzoic acid) | - |
Brevibacterium linens BL2 | |
5-5'-dithiobis-(2-nitrobenzoic acid) | - |
Trichomonas vaginalis | |
aminoethyloxyvinylglycine | - |
Achromobacter starkeyi | |
aminoethyloxyvinylglycine | - |
Aeromonas sp. | |
aminoethyloxyvinylglycine | - |
Aspergillus flavipes | |
aminoethyloxyvinylglycine | - |
Aspergillus sp. | |
aminoethyloxyvinylglycine | - |
Brevibacterium linens BL2 | |
aminoethyloxyvinylglycine | - |
Citrobacter freundii | |
aminoethyloxyvinylglycine | - |
Citrobacter intermedius | |
aminoethyloxyvinylglycine | - |
Cladosporium cladosporioides | |
aminoethyloxyvinylglycine | - |
Clostridium sporogenes | |
aminoethyloxyvinylglycine | - |
Entamoeba histolytica | |
aminoethyloxyvinylglycine | - |
Lactococcus lactis | |
aminoethyloxyvinylglycine | - |
Pseudomonas putida | |
aminoethyloxyvinylglycine | - |
Treponema denticola | |
aminoethyloxyvinylglycine | - |
Trichomonas vaginalis | |
DL-Penicillamine | - |
Brevibacterium linens BL2 | |
DL-Penicillamine | - |
Trichomonas vaginalis | |
DL-propargylglycine | - |
Brevibacterium linens BL2 | |
DL-propargylglycine | - |
Trichomonas vaginalis | |
DMSO | - |
Aspergillus flavipes | |
DMSO | - |
Lactococcus lactis | |
EDTA | - |
Aspergillus flavipes | |
hydroxylamine | - |
Brevibacterium linens BL2 | |
hydroxylamine | - |
Lactococcus lactis | |
hydroxylamine | - |
Trichomonas vaginalis | |
iodoacetamide | - |
Brevibacterium linens BL2 | |
iodoacetamide | - |
Trichomonas vaginalis | |
iodoacetate | - |
Achromobacter starkeyi | |
iodoacetic acid | - |
Aspergillus flavipes | |
iodoacetic acid | - |
Lactococcus lactis | |
L-Cycloserine | - |
Brevibacterium linens BL2 | |
L-Cycloserine | - |
Trichomonas vaginalis | |
p-chloromercuri-benzoic acid | - |
Achromobacter starkeyi | |
p-chloromercuri-benzoic acid | - |
Trichomonas vaginalis | |
p-chloromercuribenzoic acid | - |
Brevibacterium linens BL2 | |
propargylglycine | - |
Achromobacter starkeyi | |
propargylglycine | - |
Aeromonas sp. | |
propargylglycine | - |
Aspergillus flavipes | |
propargylglycine | - |
Aspergillus sp. | |
propargylglycine | - |
Brevibacterium linens BL2 | |
propargylglycine | - |
Citrobacter freundii | |
propargylglycine | - |
Citrobacter intermedius | |
propargylglycine | - |
Cladosporium cladosporioides | |
propargylglycine | - |
Clostridium sporogenes | |
propargylglycine | - |
Entamoeba histolytica | |
propargylglycine | - |
Lactococcus lactis | |
propargylglycine | - |
Pseudomonas putida | |
propargylglycine | - |
Treponema denticola | |
propargylglycine | - |
Trichomonas vaginalis | |
Semicarbazide | - |
Achromobacter starkeyi | |
Sodium arsenate | - |
Achromobacter starkeyi | |
Sodium azide | - |
Aspergillus flavipes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
L-methionine | values between 0.8 to 1.7, depending on publications | Pseudomonas putida | |
0.0013 | - |
L-methionine | - |
Aspergillus sp. | |
0.1 | - |
trifluoromethionine | - |
Entamoeba histolytica | |
0.18 | - |
L-cysteine | - |
Citrobacter freundii | |
0.2 | - |
L-ethionine | - |
Pseudomonas putida | |
0.27 | - |
L-cystathionine | - |
Lactococcus lactis | |
0.45 | - |
L-ethionine | - |
Pseudomonas putida | |
0.46 | - |
L-cysteine | - |
Lactococcus lactis | |
0.5 | - |
DL-homocysteine | - |
Trichomonas vaginalis | |
0.5 | - |
L-ethionine | - |
Trichomonas vaginalis | |
0.53 | - |
L-cysteine | - |
Pseudomonas putida | |
0.55 | - |
L-methionine | - |
Treponema denticola | |
0.56 | - |
L-ethionine | - |
Citrobacter freundii | |
0.61 | - |
L-methionine | - |
Entamoeba histolytica | |
0.61 | - |
S-methyl-L-cysteine | - |
Citrobacter freundii | |
0.64 | - |
L-cysteine | - |
Entamoeba histolytica | |
0.7 | - |
L-methionine | - |
Citrobacter freundii | |
0.77 | - |
S-methylcysteine | - |
Pseudomonas putida | |
0.87 | - |
L-methionine | - |
Lactococcus lactis | |
1.1 | - |
DL-homocysteine | - |
Citrobacter freundii | |
1.13 | - |
L-methionine | - |
Citrobacter intermedius | |
1.33 | - |
L-methionine | - |
Pseudomonas putida | |
1.8 | - |
DL-homocysteine | - |
Pseudomonas putida | |
3.03 | - |
DL-homocysteine | - |
Entamoeba histolytica | |
3.6 | - |
L-cysteine | - |
Trichomonas vaginalis | |
4.3 | - |
L-methionine | - |
Trichomonas vaginalis | |
6.43 | - |
L-homocysteine | - |
Pseudomonas putida | |
30 | - |
L-homoserine | - |
Pseudomonas putida | |
90 | - |
L-methionine | - |
Clostridium sporogenes |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Entamoeba histolytica | 5622 | - |
intracellular | - |
Lactococcus lactis | 5622 | - |
intracellular | - |
Pseudomonas putida | 5622 | - |
intracellular | - |
Trichomonas vaginalis | 5622 | - |
intracellular | - |
Citrobacter freundii | 5622 | - |
intracellular | - |
Citrobacter intermedius | 5622 | - |
intracellular | - |
Clostridium sporogenes | 5622 | - |
intracellular | - |
Aeromonas sp. | 5622 | - |
intracellular | - |
Aspergillus sp. | 5622 | - |
intracellular | - |
Treponema denticola | 5622 | - |
intracellular | - |
Cladosporium cladosporioides | 5622 | - |
intracellular | - |
Aspergillus flavipes | 5622 | - |
intracellular | - |
Achromobacter starkeyi | 5622 | - |
intracellular | - |
Brevibacterium linens BL2 | 5622 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | significant enzyme inhibition | Aspergillus flavipes | |
Co2+ | slightly stimulatory effect on the enzyme activity | Aspergillus flavipes | |
Cu2+ | slightly stimulatory effect on the enzyme activity | Aspergillus flavipes | |
Li+ | significant enzyme inhibition | Aspergillus flavipes | |
Mg2+ | slightly stimulatory effect on the enzyme activity | Aspergillus flavipes | |
Mn2+ | slightly stimulatory effect on the enzyme activity | Aspergillus flavipes | |
Na+ | significant enzyme inhibition | Aspergillus flavipes | |
Ni2+ | slightly stimulatory effect on the enzyme activity | Aspergillus flavipes |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37500 | - |
4 * 37500, SDS-PAGE | Clostridium sporogenes |
41000 | - |
4 * 41000, SDS-PAGE | Aeromonas sp. |
42600 | - |
4 * 42600, SDS-PAGE | Pseudomonas putida |
43000 | - |
4 * 43000, SDS-PAGE | Entamoeba histolytica |
43000 | - |
4 * 43000, SDS-PAGE | Citrobacter freundii |
43000 | - |
4 * 43000, SDS-PAGE | Brevibacterium linens BL2 |
44000 | - |
4 * 44000, SDS-PAGE | Trichomonas vaginalis |
45000 | - |
4 * 45000, SDS-PAGE | Pseudomonas putida |
55000 | - |
4 * 55000, SDS-PAGE | Lactococcus lactis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Achromobacter starkeyi | - |
- |
- |
Aeromonas sp. | - |
- |
- |
Aspergillus flavipes | - |
- |
- |
Aspergillus sp. | - |
- |
- |
Aspergillus sp. Rs-1a | - |
- |
- |
Brevibacterium linens BL2 | - |
- |
- |
Citrobacter freundii | - |
- |
- |
Citrobacter intermedius | - |
- |
- |
Cladosporium cladosporioides | - |
- |
- |
Clostridium sporogenes | - |
- |
- |
Entamoeba histolytica | - |
- |
- |
Lactococcus lactis | - |
- |
- |
no activity in mammalia | - |
- |
- |
Pseudomonas putida | - |
- |
- |
Treponema denticola | - |
- |
- |
Trichomonas vaginalis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ion exchange and gel filtration chromatography | Entamoeba histolytica |
ion exchange and gel filtration chromatography | Lactococcus lactis |
ion exchange and gel filtration chromatography | Pseudomonas putida |
ion exchange and gel filtration chromatography | Trichomonas vaginalis |
ion exchange and gel filtration chromatography | Citrobacter freundii |
ion exchange and gel filtration chromatography | Citrobacter intermedius |
ion exchange and gel filtration chromatography | Clostridium sporogenes |
ion exchange and gel filtration chromatography | Aeromonas sp. |
ion exchange and gel filtration chromatography | Aspergillus sp. |
ion exchange and gel filtration chromatography | Treponema denticola |
ion exchange and gel filtration chromatography | Cladosporium cladosporioides |
ion exchange and gel filtration chromatography | Aspergillus flavipes |
ion exchange and gel filtration chromatography | Brevibacterium linens BL2 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
value is 0.04 U/mg protein | Lactococcus lactis |
additional information | - |
value is 0.26 U/mg protein | Citrobacter intermedius |
additional information | - |
value is 0.44 U/mg protein | Entamoeba histolytica |
additional information | - |
value is 1.8 U/mg protein | Pseudomonas putida |
additional information | - |
value is 10 U/mg protein | Citrobacter freundii |
additional information | - |
value is 228.9 U/mg protein | Brevibacterium linens BL2 |
additional information | - |
value is 3.17 U/mg protein | Trichomonas vaginalis |
additional information | - |
value is 31.0 U/mg protein | Aeromonas sp. |
additional information | - |
value is 36.6 U/mg protein | Treponema denticola |
additional information | - |
value is 44.8 U/mg protein | Aspergillus sp. |
additional information | - |
value is 45.3 U/mg protein | Pseudomonas putida |
additional information | - |
value is 61 U/mg protein | Aspergillus flavipes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cystathionine + ? | - |
Brevibacterium linens BL2 | ? | - |
? | |
DL-ethionine + H2O | - |
Brevibacterium linens BL2 | ? | - |
? | |
DL-homocysteic acid + H2O | - |
Trichomonas vaginalis | ? | - |
? | |
DL-homocysteine + H2O | - |
Entamoeba histolytica | ? | - |
? | |
DL-homocysteine + H2O | - |
Pseudomonas putida | ? | - |
? | |
DL-homocysteine + H2O | - |
Trichomonas vaginalis | ? | - |
? | |
DL-homocysteine + H2O | - |
Citrobacter freundii | ? | - |
? | |
DL-homocysteine + H2O | - |
Clostridium sporogenes | ? | - |
? | |
DL-homocysteine + H2O | - |
Aspergillus sp. | ? | - |
? | |
DL-homocysteine + H2O | - |
Brevibacterium linens BL2 | ? | - |
? | |
DL-homocysteine + H2O | - |
Aspergillus sp. Rs-1a | ? | - |
? | |
L-cystathionine + ? | - |
Lactococcus lactis | ? | - |
? | |
L-cysteine + H2O | - |
Entamoeba histolytica | ? | - |
? | |
L-cysteine + H2O | - |
Lactococcus lactis | ? | - |
? | |
L-cysteine + H2O | - |
Pseudomonas putida | ? | - |
? | |
L-cysteine + H2O | - |
Trichomonas vaginalis | ? | - |
? | |
L-cysteine + H2O | - |
Citrobacter freundii | ? | - |
? | |
L-cysteine + H2O | - |
Clostridium sporogenes | ? | - |
? | |
L-cysteine + H2O | - |
Brevibacterium linens BL2 | ? | - |
? | |
L-ethionine + H2O | - |
Pseudomonas putida | ? | - |
? | |
L-ethionine + H2O | - |
Trichomonas vaginalis | ? | - |
? | |
L-ethionine + H2O | - |
Citrobacter freundii | ? | - |
? | |
L-ethionine + H2O | - |
Clostridium sporogenes | ? | - |
? | |
L-ethionine + H2O | - |
Aeromonas sp. | ? | - |
? | |
L-ethionine + H2O | - |
Aspergillus sp. | ? | - |
? | |
L-ethionine + H2O | - |
Aspergillus sp. Rs-1a | ? | - |
? | |
L-homocysteine + H2O | - |
Pseudomonas putida | ? | - |
? | |
L-homocysteine + H2O | - |
Aeromonas sp. | ? | - |
? | |
L-homoserine + H2O | - |
Pseudomonas putida | ? | - |
? | |
L-homoserine + H2O | - |
Aspergillus sp. | ? | - |
? | |
L-homoserine + H2O | - |
Aspergillus sp. Rs-1a | ? | - |
? | |
L-methionine + H2O | - |
Entamoeba histolytica | ? | - |
? | |
L-methionine + H2O | - |
Lactococcus lactis | ? | - |
? | |
L-methionine + H2O | - |
Pseudomonas putida | ? | - |
? | |
L-methionine + H2O | - |
Trichomonas vaginalis | ? | - |
? | |
L-methionine + H2O | - |
Citrobacter freundii | ? | - |
? | |
L-methionine + H2O | - |
Citrobacter intermedius | ? | - |
? | |
L-methionine + H2O | - |
Clostridium sporogenes | ? | - |
? | |
L-methionine + H2O | - |
Aeromonas sp. | ? | - |
? | |
L-methionine + H2O | - |
Aspergillus sp. | ? | - |
? | |
L-methionine + H2O | - |
Treponema denticola | ? | - |
? | |
L-methionine + H2O | - |
Cladosporium cladosporioides | ? | - |
? | |
L-methionine + H2O | - |
Aspergillus flavipes | ? | - |
? | |
L-methionine + H2O | - |
Brevibacterium linens BL2 | ? | - |
? | |
L-methionine + H2O | - |
Aspergillus sp. Rs-1a | ? | - |
? | |
L-selenomethionine + H2O | - |
Trichomonas vaginalis | ? | - |
? | |
O-acetyl-L-homoserine + H2O | - |
Aeromonas sp. | ? | - |
? | |
S-methyl-L-cysteine + H2O | - |
Citrobacter freundii | ? | - |
? | |
S-methylcysteine + H2O | - |
Pseudomonas putida | ? | - |
? | |
trifluoromethionine + H2O | - |
Entamoeba histolytica | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Citrobacter intermedius |
tetramer | - |
Aspergillus sp. |
tetramer | - |
Cladosporium cladosporioides |
tetramer | - |
Aspergillus flavipes |
tetramer | - |
Achromobacter starkeyi |
tetramer | 4 * 43000, SDS-PAGE | Entamoeba histolytica |
tetramer | 4 * 43000, SDS-PAGE | Citrobacter freundii |
tetramer | 4 * 43000, SDS-PAGE | Brevibacterium linens BL2 |
tetramer | 4 * 45000, SDS-PAGE | Pseudomonas putida |
tetramer | 4 * 41000, SDS-PAGE | Aeromonas sp. |
tetramer | 4 * 55000, SDS-PAGE | Lactococcus lactis |
tetramer | 4 * 37500, SDS-PAGE | Clostridium sporogenes |
tetramer | 4 * 44000, SDS-PAGE | Trichomonas vaginalis |
tetramer | 4 * 42600, SDS-PAGE | Pseudomonas putida |
tetramer | 4*43500, SDS-PAGE | Treponema denticola |
Synonyms | Comment | Organism |
---|---|---|
L-methioninase | - |
Entamoeba histolytica |
L-methioninase | - |
Lactococcus lactis |
L-methioninase | - |
Pseudomonas putida |
L-methioninase | - |
Trichomonas vaginalis |
L-methioninase | - |
Citrobacter freundii |
L-methioninase | - |
Citrobacter intermedius |
L-methioninase | - |
Clostridium sporogenes |
L-methioninase | - |
Aeromonas sp. |
L-methioninase | - |
Aspergillus sp. |
L-methioninase | - |
Treponema denticola |
L-methioninase | - |
Cladosporium cladosporioides |
L-methioninase | - |
Aspergillus flavipes |
L-methioninase | - |
Achromobacter starkeyi |
L-methioninase | - |
Brevibacterium linens BL2 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Brevibacterium linens BL2 |
30 | - |
- |
Lactococcus lactis |
30 | - |
- |
Citrobacter freundii |
30 | - |
- |
Citrobacter intermedius |
30 | - |
- |
Cladosporium cladosporioides |
30 | - |
- |
Aspergillus flavipes |
35 | - |
- |
Aspergillus sp. |
37 | - |
- |
Entamoeba histolytica |
37 | - |
- |
Pseudomonas putida |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
L-methionine | values between 25.3 to 48.6, depending on publications | Pseudomonas putida | |
0.81 | - |
trifluoromethionine | - |
Entamoeba histolytica | |
1.4 | - |
L-homoserine | - |
Pseudomonas putida | |
1.59 | - |
L-cysteine | - |
Entamoeba histolytica | |
1.82 | - |
L-methionine | - |
Entamoeba histolytica | |
2.27 | - |
L-cysteine | - |
Pseudomonas putida | |
2.3 | - |
L-cysteine | - |
Citrobacter freundii | |
3.92 | - |
DL-homocysteine | - |
Entamoeba histolytica | |
5.1 | - |
DL-homocysteine | - |
Citrobacter freundii | |
5.9 | - |
S-methyl-L-cysteine | - |
Citrobacter freundii | |
6.2 | - |
L-ethionine | - |
Citrobacter freundii | |
6.5 | - |
L-methionine | - |
Citrobacter freundii | |
33.4 | - |
L-ethionine | - |
Pseudomonas putida | |
44.8 | - |
DL-homocysteine | - |
Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Entamoeba histolytica |
7.2 | - |
- |
Pseudomonas putida |
7.5 | - |
- |
Brevibacterium linens BL2 |
8 | - |
- |
Pseudomonas putida |
8 | - |
- |
Citrobacter freundii |
8 | - |
- |
Aeromonas sp. |
8 | - |
- |
Aspergillus sp. |
8 | - |
- |
Aspergillus flavipes |
9 | - |
- |
Cladosporium cladosporioides |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Trichomonas vaginalis |
7.5 | 8.5 | - |
Clostridium sporogenes |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.6 | 8.2 | - |
Entamoeba histolytica |
6 | 9 | - |
Pseudomonas putida |
6 | 9 | - |
Aeromonas sp. |
6 | 8 | - |
Brevibacterium linens BL2 |
6 | 10 | - |
Pseudomonas putida |
6.5 | 9 | - |
Trichomonas vaginalis |
8 | 9 | - |
Aspergillus sp. |
8 | 9 | - |
Aspergillus flavipes |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Entamoeba histolytica | |
pyridoxal 5'-phosphate | - |
Lactococcus lactis | |
pyridoxal 5'-phosphate | - |
Pseudomonas putida | |
pyridoxal 5'-phosphate | - |
Trichomonas vaginalis | |
pyridoxal 5'-phosphate | - |
Citrobacter freundii | |
pyridoxal 5'-phosphate | - |
Citrobacter intermedius | |
pyridoxal 5'-phosphate | - |
Clostridium sporogenes | |
pyridoxal 5'-phosphate | - |
Aeromonas sp. | |
pyridoxal 5'-phosphate | - |
Aspergillus sp. | |
pyridoxal 5'-phosphate | - |
Treponema denticola | |
pyridoxal 5'-phosphate | - |
Cladosporium cladosporioides | |
pyridoxal 5'-phosphate | - |
Aspergillus flavipes | |
pyridoxal 5'-phosphate | - |
Achromobacter starkeyi | |
pyridoxal 5'-phosphate | - |
Brevibacterium linens BL2 |