Crystallization (Comment) | Organism |
---|---|
1.8 A resolution. Residues Y59 and R61 of neighbouring subunits contact the phosphate group of pyridoxal 5-phosphate. Residues K240, D241 and R61 of one partner monomer and Y114 and C116 of the other form a hydrogen-bond network in the active site | Pseudomonas putida |
Protein Variants | Comment | Organism |
---|---|---|
C116S | 9% of wild-type activity | Pseudomonas putida |
C116T | 40% of wild-type activity | Pseudomonas putida |
F128C | 112% of wild-type activity | Pseudomonas putida |
G9C/D385C | 46% of wild-type activity | Pseudomonas putida |
L341H | 9% of wild-type activity | Pseudomonas putida |
R61A | 1% of wild-type activity | Pseudomonas putida |
R61E | no activity | Pseudomonas putida |
R61F | 2% of wild-type activity | Pseudomonas putida |
S248C | 131% of wild-type activity | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | P13254 | - |
- |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Pseudomonas putida |